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Literature summary for 1.12.7.2 extracted from

  • Eckert, C.A.; Freed, E.; Wawrousek, K.; Smolinski, S.; Yu, J.; Maness, P.C.
    Inactivation of the uptake hydrogenase in the purple non-sulfur photosynthetic bacterium Rubrivivax gelatinosus CBS enables a biological water-gas shift platform for H2 production (2019), J. Ind. Microbiol. Biotechnol., 46, 993-1002 .
    View publication on PubMed
Search for more literature for 1.12.7.2

Organism

Organism UniProt Comment Textmining
Rubrivivax gelatinosus
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Rubrivivax gelatinosus CBS
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Synonyms

Synonyms Comment Organism
CooH gene name, ec 1.12.7.2 Rubrivivax gelatinosus
Ech hydrogenase ec 1.12.7.2 Rubrivivax gelatinosus
energy-converting hydrogenase
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 Rubrivivax gelatinosus
MBH hydrogenase ec 1.12.7.2 Rubrivivax gelatinosus

Expression

Organism Comment Expression
Rubrivivax gelatinosus when CO was present, expression of all tested hyp1 genes (hypA1 and hypE1) is induced along with the cooH gene, which encodes the catalytic subunit of the Ech hydrogenase. Induction under H2 is not apparent for the hyp1 and cooH genes up

General Information

General Information Comment Organism
malfunction Ech H2 evolving activity is only delayed when hyp1 is deleted. Deletion of hyp1FCDE alone still results in H2 production and CO and H2 uptake, albeit in a delayed manner compared to wild-type Rubrivivax gelatinosus
malfunction hyp2 deletion completely disrupts MBH H2 uptake. When both hyp1FCDE and hyp2 are deleted (hyp1/2DELTA), there is no significant CO uptake and no H2 detected, indicating that the maturation and activity of both hydrogenases is likely disrupted. At least some of the absent Hyp2 proteins, and not HypA1 and HypB1 alone, are required for rescue of maturation of the Ech hydrogenase Rubrivivax gelatinosus