Protein Variants | Comment | Organism |
---|---|---|
additional information | engineering and expressing of a His-tagged minimal catalytically active 4-subunit cytoplasmic subcomplex of respiratory membrane-bound hydrogenase. In order to obtain a minimal subcomplex, the native MBH operon is splitt it into two separate transcriptional units | Pyrococcus furiosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | minimal catalytically active 4-subunit subcomplex of respiratory membrane-bound hydrogenase | Pyrococcus furiosus | 5737 | - |
membrane | native 14-subunit membrane-bound subcomplex of respiratory membrane-bound hydrogenase | Pyrococcus furiosus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | contains [NiFe]-clusters. The Fe: Ni ratio of the engineered and expressed His-tagged 4-subunit subcomplex of respiratory membrane-bound hydrogenase. Subunit MbhL harbors the [NiFe] active site, subunit MbhK is part of the large subunit, subunit MbhJ harbors one [4Fe4S] cluster and MbhN harbors two [4Fe4S] clusters | Pyrococcus furiosus | |
Ni | contains [NiFe]-clusters. The Fe: Ni ratio of the engineered and expressed His-tagged 4-subunit subcomplex of respiratory membrane-bound hydrogenase. Subunit MbhL harbors the [NiFe] active site, subunit MbhK is part of the large subunit, subunit MbhJ harbors one [4Fe4S] cluster and MbhN harbors two [4Fe4S] clusters | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
gel filtration, engineered minimal catalytically active 4-subunit subcomplex of respiratory membrane-bound hydrogenase | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q8U0Z8 and Q8U0Z7 and Q8U0Z6 | Q8U0Z8: subunit mbhJ, Q8U0Z7: subunit mbhK, Q8U0Z6: catalytic subunit mbhL, subunit mbhN. Engineered minimal catalytically active 4-subunit subcomplex of respiratory membrane-bound hydrogenase, MbhJKLN | - |
Oxidation Stability | Organism |
---|---|
the O2 sensitivities of minimal catalytically active 4-subunit subcomplex of respiratory membrane-bound hydrogenase and the native 14-subunit membrane-bound version are similar, half-lives of about 15 h in air | Pyrococcus furiosus |
Purification (Comment) | Organism |
---|---|
purification of the engineered and expressed His-tagged 4-subunit cytoplasmic subcomplex of respiratory membrane-bound hydrogenase | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + 2 oxidized ferredoxin | - |
Pyrococcus furiosus | 2 reduced ferredoxin + 2 H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
C-MBH | engineered minimal catalytically active 4-subunit subcomplex of respiratory membrane-bound hydrogenase | Pyrococcus furiosus |
MbhJKLN | engineered minimal catalytically active 4-subunit subcomplex of respiratory membrane-bound hydrogenase, MbhJKLN | Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
the minimal catalytically active 4-subunit subcomplex of respiratory membrane-bound hydrogenase, H2 formation with the physiological electron donor, reduced ferredoxin | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
half-life of minimal catalytically active 4-subunit subcomplex of respiratory membrane-bound hydrogenase: 8 h. Half-life of the native 14-subunit membrane-bound respiratory membrane-bound hydrogenase complex | Pyrococcus furiosus |