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Literature summary for 1.12.7.2 extracted from

  • Meuer, J.; Bartoschek, S.; Koch, J.; Kunkel, A.; Hedderich, R.
    Purification and catalytic properties of Ech hydrogenase from Methanosarcina barkeri (1999), Eur. J. Biochem., 265, 325-335.
    View publication on PubMed
Search for more literature for 1.12.7.2

Inhibitors

Inhibitors Comment Organism Structure
O2 90% inactivation after 30 min Methanosarcina barkeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 Ferredoxin
-
 Methanosarcina barkeri
0.005
-
 H2
-
 Methanosarcina barkeri
0.0075
-
 reduced ferredoxin
-
 Methanosarcina barkeri

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Methanosarcina barkeri 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe 11.3 mol non-heme iron per mol enzyme Methanosarcina barkeri
Ni 0.9 mol Ni per mol enzyme Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2 + oxidized ferredoxin Methanosarcina barkeri
-
 H+ + reduced ferredoxin
-
 r
additional information Methanosarcina barkeri major membrane protein in acetate-grown, methanol-grown or H2/CO2-grown cells ?
-
 ?
additional information Methanosarcina barkeri Fusaro / DSM 804 major membrane protein in acetate-grown, methanol-grown or H2/CO2-grown cells ?
-
 ?

Organism

Organism UniProt Comment Textmining
Methanosarcina barkeri
-
-
-
Methanosarcina barkeri Fusaro / DSM 804
-
-
-

Oxidation Stability

Oxidation Stability Organism
rapid inactivation under aerobic conditions Methanosarcina barkeri

Purification (Commentary)

Purification (Comment) Organism
-
 Methanosarcina barkeri

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
50
-
 hydrogen oxidation, ferredoxin as electron acceptor Methanosarcina barkeri
53
-
 hydrogen oxidation, methyl viologen as electron acceptor Methanosarcina barkeri
90
-
 hydrogen evolution, ferredoxin as electron donor Methanosarcina barkeri
123
-
 hydrogen oxidation, methylene blue as electron acceptor Methanosarcina barkeri
210
-
 hydrogen oxidation, benzyl viologen as electron acceptor Methanosarcina barkeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + electron acceptor methylene blue as electron acceptor Methanosarcina barkeri H+ + reduced electron acceptor
-
 r
H2 + electron acceptor methyl viologen as electron acceptor Methanosarcina barkeri H+ + reduced electron acceptor
-
 r
H2 + oxidized ferredoxin
-
 Methanosarcina barkeri H+ + reduced ferredoxin
-
 r
additional information major membrane protein in acetate-grown, methanol-grown or H2/CO2-grown cells Methanosarcina barkeri ?
-
 ?
additional information major membrane protein in acetate-grown, methanol-grown or H2/CO2-grown cells Methanosarcina barkeri Fusaro / DSM 804 ?
-
 ?