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Literature summary for 1.12.2.1 extracted from
- Purification and properties of the soluble hydrogenase from Desulfovibrio desulfuricans (strain Norway 4) (1984), Eur. J. Biochem., 145, 637-643.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | bound to | Desulfovibrio desulfuricans | 16020 | - |
| soluble | - |
Desulfovibrio desulfuricans | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | 4.8 iron atoms per molecule | Desulfovibrio desulfuricans |  |
| Nickel | 0.45 nickel atoms per molecule | Desulfovibrio desulfuricans | |
| selenium | 0.45 selenium atoms per molecule | Desulfovibrio desulfuricans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27100 | - |
1 * 27100 + 1 * 59800, membrane-bound enzyme, SDS-PAGE | Desulfovibrio desulfuricans |
| 28600 | - |
1 * 28600 + 1 * 56400, soluble enzyme, SDS-PAGE | Desulfovibrio desulfuricans |
| 56400 | - |
1 * 28600 + 1 * 56400, soluble enzyme, SDS-PAGE | Desulfovibrio desulfuricans |
| 59800 | - |
1 * 27100 + 1 * 59800, membrane-bound enzyme, SDS-PAGE | Desulfovibrio desulfuricans |
| 61000 | - |
membrane-bound enzyme, gel filtration | Desulfovibrio desulfuricans |
| 67000 | - |
soluble enzyme, gel filtration | Desulfovibrio desulfuricans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Desulfovibrio desulfuricans | - |
Norway 4 | - |
| Desulfovibrio desulfuricans Norway 4 | - |
Norway 4 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| soluble enzyme | Desulfovibrio desulfuricans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 250 | - |
- |
Desulfovibrio desulfuricans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + ferricytochrome c3 | - |
Desulfovibrio desulfuricans | H+ + ferrocytochrome c3 | - |
? | |
| H2 + ferricytochrome c3 | - |
Desulfovibrio desulfuricans Norway 4 | H+ + ferrocytochrome c3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 28600 + 1 * 56400, soluble enzyme, SDS-PAGE | Desulfovibrio desulfuricans |
| dimer | 1 * 27100 + 1 * 59800, membrane-bound enzyme, SDS-PAGE | Desulfovibrio desulfuricans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Desulfovibrio desulfuricans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome c3 | - |
Desulfovibrio desulfuricans |
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