Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CO | reversible | Desulfovibrio desulfuricans | |
CO | reversible | Desulfovibrio vulgaris | |
CO | - |
Megalodesulfovibrio gigas | |
O2 | reversible inactivation | Desulfovibrio desulfuricans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound to | Desulfovibrio desulfuricans | 16020 | - |
periplasm | - |
Desulfovibrio desulfuricans | - |
- |
periplasm | - |
Megalodesulfovibrio gigas | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | nonheme iron protein | Megalodesulfovibrio gigas | |
Iron | 12.5 gatom nonheme iron and 11.5 gatom of acid-labile sulfide per mol, arranged in at least two Fe4S4 clusters of the ferredoxin type | Desulfovibrio vulgaris | |
Iron | presence of a three-iron center | Desulfovibrio desulfuricans | |
Iron | 11-12 gatom of nonheme iron per molecule | Megalodesulfovibrio gigas | |
Nickel | - |
Desulfovibrio desulfuricans | |
Nickel | 1 gatom of nickel per molecule | Megalodesulfovibrio gigas | |
Nickel | purified enzyme contains variable amounts of nickel, ranging from 0.1 to 0.6 gatom per mol of enzyme | Desulfovibrio vulgaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
x * 26000 + x * 62000 | Megalodesulfovibrio gigas |
58000 | - |
- |
Desulfovibrio desulfuricans |
62000 | - |
x * 26000 + x * 62000 | Megalodesulfovibrio gigas |
89500 | - |
- |
Megalodesulfovibrio gigas |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Desulfovibrio desulfuricans | - |
Norway 4 | - |
Desulfovibrio desulfuricans Norway 4 | - |
Norway 4 | - |
Desulfovibrio vulgaris | - |
Hildenborough | - |
Desulfovibrio vulgaris Hildenborough | - |
Hildenborough | - |
Megalodesulfovibrio gigas | - |
- |
- |
Oxidation Stability | Organism |
---|---|
long exposure of the oxidized enzyme to oxygen does not irreversibly inactivate the hydrogenase. In the reduced form the hydrogenase is irreveribly inactivated | Desulfovibrio vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + ferredoxin | requires the presence of cytochrome c3 for the reduction of ferredoxin | Megalodesulfovibrio gigas | H+ + reduced ferredoxin | - |
? | |
H2 + ferricytochrome c3 | - |
Desulfovibrio desulfuricans | H+ + ferrocytochrome c3 | - |
? | |
H2 + ferricytochrome c3 | - |
Desulfovibrio vulgaris | H+ + ferrocytochrome c3 | - |
? | |
H2 + ferricytochrome c3 | - |
Megalodesulfovibrio gigas | H+ + ferrocytochrome c3 | - |
? | |
H2 + ferricytochrome c3 | - |
Desulfovibrio desulfuricans Norway 4 | H+ + ferrocytochrome c3 | - |
? | |
H2 + ferricytochrome c3 | - |
Desulfovibrio vulgaris Hildenborough | H+ + ferrocytochrome c3 | - |
? | |
H2 + rubredoxin | requires the presence of cytochrome c3 for the reduction of rubredoxin | Megalodesulfovibrio gigas | H+ + reduced rubredoxin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 26000 + x * 62000 | Megalodesulfovibrio gigas |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c3 | - |
Desulfovibrio desulfuricans | |
cytochrome c3 | - |
Desulfovibrio vulgaris | |
cytochrome c3 | requires the presence of cytochrome c3 for the reduction of ferredoxin, rubredoxin and cytochrome c3 | Megalodesulfovibrio gigas |