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Literature summary for 1.12.1.2 extracted from

  • Cano, M.; Volbeda, A.; Guedeney, G.; Aubert-Jousset, E.; Richaud, P.; Peltier, G.; Cournac, L.
    Improved oxygen tolerance of the Synechocystis sp. PCC 6803 bidirectional hydrogenase by site-directed mutagenesis of putative residues of the gas diffusion channel (2014), Int. J. Hydrogen Energy, 39, 16872-16884 .
No PubMed abstract available

Protein Variants

Protein Variants Comment Organism
I64M the mutation alters gas diffusion kinetics and improves O2 tolerance Synechocystis sp.
I64M/L107M the mutant shows strongly reduced activity compared to the wild type enzyme Synechocystis sp.
I64M/L107M/L112M the mutant shows strongly reduced activity compared to the wild type enzyme Synechocystis sp.
I64M/L112M the mutant shows strongly reduced activity compared to the wild type enzyme Synechocystis sp.
L107F the mutant shows reduced activity compared to the wild type enzyme Synechocystis sp.
L107M/L112M the mutant shows reduced activity compared to the wild type enzyme Synechocystis sp.
L112F the mutant shows strongly reduced activity compared to the wild type enzyme Synechocystis sp.
L112M the mutant shows slightly reduced activity compared to the wild type enzyme Synechocystis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2 + NAD+ Synechocystis sp.
-
H+ + NADH
-
?

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + NAD+
-
Synechocystis sp. H+ + NADH
-
?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Synechocystis sp.

Synonyms

Synonyms Comment Organism
HoxH subunit Synechocystis sp.
HoxY subunit Synechocystis sp.
[NiFe] hydrogenase
-
Synechocystis sp.