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Literature summary for 1.12.1.2 extracted from

  • Germer, F.; Zebger, I.; Saggu, M.; Lendzian, F.; Schulz, R.; Appel, J.
    Overexpression, isolation and spectroscopic characterization of the bidirectional [NiFe]-hydrogenase from Synechocystis sp. PCC 6803 (2009), J. Biol. Chem., 284, 36462-36472.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

Metals/Ions Comment Organism Structure
Iron the enzyme contains at least one [4Fe4S]+ and at least one [2Fe2S]+ cluster Synechocystis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H+ + NADH Synechocystis sp.
-
H2 + NAD+
-
?
H+ + NADPH Synechocystis sp.
-
H2 + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information six accessory proteins HypA-F (hydrogenase pleiotropic proteins) and one protease (HoxW) are necessary for post-translational processing of the bidirectional [NiFe] hydrogenase Synechocystis sp.

Purification (Commentary)

Purification (Comment) Organism
Strep-Tactin-Sepharose column chromatography Synechocystis sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.076
-
crude extract, pH and temperature not specified in the publication Synechocystis sp.
87.78
-
after 1155old purification, pH and temperature not specified in the publication Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H+ + NADH
-
Synechocystis sp. H2 + NAD+
-
?
H+ + NADPH
-
Synechocystis sp. H2 + NADP+
-
?
H+ + reduced dithionite
-
Synechocystis sp. H2 + oxidized dithionite
-
?
H+ + reduced methyl viologen
-
Synechocystis sp. H2 + oxidized methyl viologen
-
?

Synonyms

Synonyms Comment Organism
bidirectional [NiFe] hydrogenase
-
Synechocystis sp.
Hox
-
Synechocystis sp.

Cofactor

Cofactor Comment Organism Structure
NADH
-
Synechocystis sp.
NADPH
-
Synechocystis sp.