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Literature summary for 1.12.1.2 extracted from
- A multisubunit membrane-bound [NiFe] hydrogenase and an NADH-dependent Fe-only hydrogenase in the fermenting bacterium Thermoanaerobacter tengcongensis (2004), Microbiology, 150, 2451-2463.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| FMN | activates | Caldanaerobacter subterraneus subsp. tengcongensis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.017 | - |
NADH | 70°C | Caldanaerobacter subterraneus subsp. tengcongensis | |
| 0.09 | - |
NAD+ | 70°C | Caldanaerobacter subterraneus subsp. tengcongensis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Caldanaerobacter subterraneus subsp. tengcongensis | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | enzyme contains multiple iron-sulfur clusters, [4Fe-4S] or [2Fe-2S] | Caldanaerobacter subterraneus subsp. tengcongensis | |
| Ni2+ | below 0.06 mol Ni2+ per mol of enzyme | Caldanaerobacter subterraneus subsp. tengcongensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 14000 | - |
1 * 65000 + 1 * 64000 + 1 * 20000 + 1 * 14000, SDS-PAGE | Caldanaerobacter subterraneus subsp. tengcongensis |
| 20000 | - |
1 * 65000 + 1 * 64000 + 1 * 20000 + 1 * 14000, SDS-PAGE | Caldanaerobacter subterraneus subsp. tengcongensis |
| 64000 | - |
1 * 65000 + 1 * 64000 + 1 * 20000 + 1 * 14000, SDS-PAGE | Caldanaerobacter subterraneus subsp. tengcongensis |
| 65000 | - |
1 * 65000 + 1 * 64000 + 1 * 20000 + 1 * 14000, SDS-PAGE | Caldanaerobacter subterraneus subsp. tengcongensis |
| 162500 | - |
amino acid sequence calculation | Caldanaerobacter subterraneus subsp. tengcongensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + NAD+ | Caldanaerobacter subterraneus subsp. tengcongensis | - |
H+ + NADH | - |
r | |
| additional information | Caldanaerobacter subterraneus subsp. tengcongensis | at high H2 partial pressure enzyme activity is reduced and fermentation is partially shifted to ethanol production | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caldanaerobacter subterraneus subsp. tengcongensis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 20fold by several chromatographic steps | Caldanaerobacter subterraneus subsp. tengcongensis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | activity is high in cells grown at low H2 partial pressure | Caldanaerobacter subterraneus subsp. tengcongensis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.6 | - |
H2-evolution in vivo, fermenter-grown cells | Caldanaerobacter subterraneus subsp. tengcongensis |
| 5 | - |
purified enzyme, H2 consumption | Caldanaerobacter subterraneus subsp. tengcongensis |
| 10 | - |
purified enzyme, H2 production | Caldanaerobacter subterraneus subsp. tengcongensis |
| 1300 | - |
purified enzyme, with substrates oxidized benzyl viologen and NADH | Caldanaerobacter subterraneus subsp. tengcongensis |
| 1700 | - |
purified enzyme, both reaction directions with substrates oxidized/reduced methyl viologen and H2/H+ | Caldanaerobacter subterraneus subsp. tengcongensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + NAD+ | - |
Caldanaerobacter subterraneus subsp. tengcongensis | H+ + NADH | - |
r | |
| H2 + NAD+ | electron transfer between the catalytic site for NADH-oxidation and the hydrogenase catalytic site is rate-limiting | Caldanaerobacter subterraneus subsp. tengcongensis | H+ + NADH | - |
r | |
| H2 + oxidized methyl viologen | - |
Caldanaerobacter subterraneus subsp. tengcongensis | H+ + reduced methyl viologen | - |
r | |
| additional information | at high H2 partial pressure enzyme activity is reduced and fermentation is partially shifted to ethanol production | Caldanaerobacter subterraneus subsp. tengcongensis | ? | - |
? | |
| additional information | enzyme autoxidation | Caldanaerobacter subterraneus subsp. tengcongensis | ? | - |
? | |
| NADH + oxidized benzyl viologen | - |
Caldanaerobacter subterraneus subsp. tengcongensis | NAD+ + reduced benzyl viologen | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 1 * 65000 + 1 * 64000 + 1 * 20000 + 1 * 14000, SDS-PAGE | Caldanaerobacter subterraneus subsp. tengcongensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NADH-dependent Fe-only hydrogenase | - |
Caldanaerobacter subterraneus subsp. tengcongensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
assay at | Caldanaerobacter subterraneus subsp. tengcongensis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | required, enzyme contains 1 mol FMN per mol of enzyme | Caldanaerobacter subterraneus subsp. tengcongensis | |
| additional information | methyl viologen or benzyl viologen can replace NAD+/NADH | Caldanaerobacter subterraneus subsp. tengcongensis | |
| additional information | no activity with NADPH/NADP+ | Caldanaerobacter subterraneus subsp. tengcongensis | |
| NAD+ | highly specific for | Caldanaerobacter subterraneus subsp. tengcongensis | |
| NADH | highly specific for | Caldanaerobacter subterraneus subsp. tengcongensis | |
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