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Literature summary for 1.11.2.4 extracted from

  • Girhard, M.; Schuster, S.; Dietrich, M.; Durre, P.; Urlacher, V.B.
    Cytochrome P450 monooxygenase from Clostridium acetobutylicum: a new alpha-fatty acid hydroxylase (2007), Biochem. Biophys. Res. Commun., 362, 114-119.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Clostridium acetobutylicum

Inhibitors

Inhibitors Comment Organism Structure
H2O2 although a high concentration of hydrogen peroxide of 0.2 mM is necessary for high activities of the enzyme, it leads to a fast enzyme inactivation within 2-4 min Clostridium acetobutylicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
SDS-PAGE Clostridium acetobutylicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Clostridium acetobutylicum CYP152A2 has a clear preference for hydroxylation at alpha-position ?
-
?
myristic acid + H2O2 Clostridium acetobutylicum
-
?
-
?

Organism

Organism UniProt Comment Textmining
Clostridium acetobutylicum Q97DZ0
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Clostridium acetobutylicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lauric acid + H2O2 after 2 min of reaction conversion of 0.06 mM lauric acid reaches 55% Clostridium acetobutylicum ?
-
?
additional information CYP152A2 has a clear preference for hydroxylation at alpha-position Clostridium acetobutylicum ?
-
?
additional information even chain fatty acids with chain length between C10 and C16 are the substrates for P450CLA Clostridium acetobutylicum ?
-
?
additional information the initial activities of P450CLA in reconstitution systems based on the use of oxygen and NADPH are either much lower than those measured with H2O2 or not measurable at all, no conversion is detected for caprylic acid and stearic acid Clostridium acetobutylicum ?
-
?
myristic acid + H2O2
-
Clostridium acetobutylicum ?
-
?
myristic acid + H2O2 highest activity, after 2 min of reaction conversion of 0.06 mM myristic acid reaches 40% Clostridium acetobutylicum ?
-
?
palmitic acid + H2O2 after 2 min of reaction conversion of 0.06 mM palmitic acid reaches 60% Clostridium acetobutylicum ?
-
?

Subunits

Subunits Comment Organism
? x * 48000, SDS-PAGE Clostridium acetobutylicum

Synonyms

Synonyms Comment Organism
alpha-fatty acid hydroxylase
-
Clostridium acetobutylicum
CYP152A2
-
Clostridium acetobutylicum
cytochrome P450 monooxygenase
-
Clostridium acetobutylicum
P450CLA
-
Clostridium acetobutylicum

General Information

General Information Comment Organism
physiological function CYP152A2 is a rapid hydrogen peroxide scavenging enzyme Clostridium acetobutylicum