Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris and Escherichia coli | Avena sativa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | associated with membrane | Avena sativa | 16020 | - |
microsome | - |
Avena sativa | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Avena sativa | G1JSL4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-cis-heptadecenoic acid + cumene hydroperoxide | 94% of the activity with oleic acid | Avena sativa | 10,11-epoxyheptadecanoic acid + ? | - |
? | |
10-cis-pentadecenoic acid + cumene hydroperoxide | 98% of the activity with oleic acid | Avena sativa | 10,11-epoxypentadecanoic acid + ? | - |
? | |
9-cis-hexadecenoic acid + cumene hydroperoxide | 89% of the activity with oleic acid | Avena sativa | 9,10-epoxy hexadecanoic acid + ? | - |
? | |
linoleic acid + cumene hydroperoxide | 47% of the activity with oleic acid | Avena sativa | ? | - |
? | |
additional information | isoform PXG1 catalyzes the strictly hydroperoxide-dependent epoxidation of unsaturated fatty acids. It prefers hydroperoxy-trienoic acids over hydroperoxydienoic acids as oxygen donors to oxidize a wide range of unsaturated fatty acids with cis double bonds. Phospholipids and acyl-CoA are not effective substrates and it will only use free fatty acid or fatty acid methyl esters as substrates. Both hydroperoxy-dienoic and hydroperoxy-trienoic acids are effectively utilized as oxygen donors, with hydroperoxy-trienoic acids being more efficiently reduced | Avena sativa | ? | - |
? | |
oleic acid + cumene hydroperoxide | preferred substrate | Avena sativa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PXG1 | - |
Avena sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Avena sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Avena sativa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | - |
Avena sativa |