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Literature summary for 1.11.2.1 extracted from

  • Aranda, C.; Municoy, M.; Guallar, V.; Kiebist, J.; Scheibner, K.; Ullrich, R.; Del Rio, J.; Hofrichter, M.; Martinez, A.; Gutierrez, A.
    Selective synthesis of 4-hydroxyisophorone and 4-ketoisophorone by fungal peroxygenases (2019), Catal. Sci. Technol., 9, 1398-1405 .
No PubMed abstract available
Search for more literature for 1.11.2.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.309
-
 isophorone at pH 7.0 and 30°C Chaetomium globosum
0.633
-
 isophorone at pH 7.0 and 30°C Humicola insolens
1.38
-
 isophorone at pH 7.0 and 30°C Cyclocybe aegerita

Organism

Organism UniProt Comment Textmining
Chaetomium globosum
-
-
-
Chaetomium globosum DSM 62110
-
-
-
Coprinopsis cinerea
-
-
-
Cyclocybe aegerita B9W4V6
-
-
Cyclocybe aegerita TM-A1 B9W4V6
-
-
Humicola insolens
-
-
-
Marasmius rotula A0A023H437
-
-
Marasmius rotula DSM 25031 A0A023H437
-
-

Purification (Commentary)

Purification (Comment) Organism
ion exchange chromatography and gel filtration Coprinopsis cinerea
ion exchange chromatography and gel filtration Humicola insolens
ion exchange chromatography and gel filtration Chaetomium globosum
ion exchange chromatography and gel filtration Cyclocybe aegerita
ion exchange chromatography and gel filtration Marasmius rotula

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isophorone + H2O2 72% conversion, reaction with 0.0001 mM enzyme and 5 mM H2O2 Cyclocybe aegerita (S)-4-hydroxyisophorone + 7-hydroxyisophorone + 7-formylisophorone + 4-ketoisophorone + H2O
-
 ?
isophorone + H2O2 96% conversion, reaction with 0.00025 mM enzyme and 5 mM H2O2 Cyclocybe aegerita (S)-4-hydroxyisophorone + 7-hydroxyisophorone + 7-formylisophorone + 4-ketoisophorone + H2O
-
 ?
isophorone + H2O2 72% conversion, reaction with 0.0001 mM enzyme and 5 mM H2O2 Cyclocybe aegerita TM-A1 (S)-4-hydroxyisophorone + 7-hydroxyisophorone + 7-formylisophorone + 4-ketoisophorone + H2O
-
 ?
isophorone + H2O2 96% conversion, reaction with 0.00025 mM enzyme and 5 mM H2O2 Cyclocybe aegerita TM-A1 (S)-4-hydroxyisophorone + 7-hydroxyisophorone + 7-formylisophorone + 4-ketoisophorone + H2O
-
 ?
isophorone + H2O2 84% conversion, reaction with 0.0001 mM enzyme and 2.5 mM H2O2 Humicola insolens 4-hydroxyisophorone + 4-ketoisophorone + 7-formylisophorone + H2O
-
 ?
isophorone + H2O2 100% conversion, reaction with 0.0005 mM enzyme and 5 mM H2O2 Humicola insolens 4-hydroxyisophorone + 4-ketoisophorone + H2O
-
 ?
isophorone + H2O2 95% conversion, reaction with 0.0001 mM enzyme and 2.5 mM H2O2 Chaetomium globosum 4-hydroxyisophorone + 4-ketoisophorone + H2O
-
 ?
isophorone + H2O2 95% conversion, reaction with 0.0001 mM enzyme and 2.5 mM H2O2 Chaetomium globosum DSM 62110 4-hydroxyisophorone + 4-ketoisophorone + H2O
-
 ?
isophorone + H2O2 100% conversion, reaction with 0.0005 mM enzyme and 5 mM H2O2 Chaetomium globosum 4-ketoisophorone + H2O
-
 ?
isophorone + H2O2 100% conversion, reaction with 0.0005 mM enzyme and 5 mM H2O2 Chaetomium globosum DSM 62110 4-ketoisophorone + H2O
-
 ?
additional information the enzyme fails to transform isophorone Coprinopsis cinerea ?
-
-
additional information the enzyme fails to transform isophorone Marasmius rotula ?
-
-
additional information the enzyme fails to transform isophorone Marasmius rotula DSM 25031 ?
-
-

Subunits

Subunits Comment Organism
? x * 46000, SDS-PAGE Cyclocybe aegerita
? x * 32000, SDS-PAGE Marasmius rotula
? x * 44000, SDS-PAGE Coprinopsis cinerea

Synonyms

Synonyms Comment Organism
UPO
-
 Coprinopsis cinerea
UPO
-
 Humicola insolens
UPO
-
 Chaetomium globosum
UPO
-
 Cyclocybe aegerita
UPO
-
 Marasmius rotula

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4
-
 isophorone at pH 7.0 and 30°C Chaetomium globosum
20.1
-
 isophorone at pH 7.0 and 30°C Cyclocybe aegerita
42
-
 isophorone at pH 7.0 and 30°C Humicola insolens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
14.2
-
 isophorone at pH 7.0 and 30°C Chaetomium globosum
14.6
-
 isophorone at pH 7.0 and 30°C Cyclocybe aegerita
66.4
-
 isophorone at pH 7.0 and 30°C Humicola insolens