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Literature summary for 1.11.2.1 extracted from

  • Aranda, C.; Ullrich, R.; Kiebist, J.; Scheibner, K.; Del Rio, J.; Hofrichter, M.; Martinez, A.; Gutierrez, A.
    Selective synthesis of the resveratrol analogue 4,4'-dihydroxy-trans-stilbene and stilbenoids modification by fungal peroxygenases (2018), Catal. Sci. Technol., 8, 2394-2401 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Aspergillus oryzae Coprinopsis cinerea
expressed in Aspergillus oryzae Cyclocybe aegerita

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0141
-
4-hydroxy-trans-stilbene at pH 7.0 and 24°C Cyclocybe aegerita
0.0153
-
trans-stilbene at pH 7.0 and 24°C Marasmius rotula
0.0189
-
4-hydroxy-trans-stilbene at pH 7.0 and 24°C Coprinopsis cinerea
0.0208
-
trans-stilbene at pH 7.0 and 24°C Cyclocybe aegerita
0.0305
-
trans-stilbene at pH 7.0 and 24°C Coprinopsis cinerea
0.0464
-
4-hydroxy-trans-stilbene at pH 7.0 and 24°C Marasmius rotula

Organism

Organism UniProt Comment Textmining
Chaetomium globosum
-
-
-
Coprinopsis cinerea
-
-
-
Cyclocybe aegerita B9W4V6
-
-
Marasmius rotula A0A023H437
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography, SP Sepharose column chromatography, and Mono-S ion-exchange column chromatography Cyclocybe aegerita
S-Sepharose column chromatography and SP-Sepharose column chromatography Coprinopsis cinerea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxy-trans-stilbene + H2O2
-
Coprinopsis cinerea 4,4'-dihydroxy-trans-stilbene + H2O
-
?
4-hydroxy-trans-stilbene + H2O2
-
Chaetomium globosum 4,4'-dihydroxy-trans-stilbene + H2O
-
?
4-hydroxy-trans-stilbene + H2O2
-
Cyclocybe aegerita 4,4'-dihydroxy-trans-stilbene + H2O
-
?
4-hydroxy-trans-stilbene + H2O2
-
Marasmius rotula 4,4'-dihydroxy-trans-stilbene + H2O
-
?
additional information no activity with pinosylvin and resveratrol Chaetomium globosum ?
-
-
pinosylvin + H2O2
-
Coprinopsis cinerea oxyresveratrol + oxypinosylvin + resveratrol + H2O
-
?
pinosylvin + H2O2
-
Cyclocybe aegerita oxyresveratrol + resveratrol + H2O
-
?
resveratrol + H2O2
-
Coprinopsis cinerea oxyresveratrol + H2O
-
?
resveratrol + H2O2
-
Cyclocybe aegerita oxyresveratrol + H2O
-
?
trans-stilbene + H2O2
-
Coprinopsis cinerea 4,4'-dihydroxy-trans-stilbene + tri-hydroxylated trans-stilbene + H2O
-
?
trans-stilbene + H2O2
-
Cyclocybe aegerita 4,4'-dihydroxy-trans-stilbene + tri-hydroxylated trans-stilbene + H2O
-
?
trans-stilbene + H2O2
-
Marasmius rotula 4,4'-dihydroxy-trans-stilbene + tri-hydroxylated trans-stilbene + H2O
-
?
trans-stilbene + H2O2
-
Chaetomium globosum trans-stilbene epoxide + H2O
-
?
veratryl alcohol + H2O2
-
Coprinopsis cinerea veratraldehyde + H2O
-
?
veratryl alcohol + H2O2
-
Chaetomium globosum veratraldehyde + H2O
-
?
veratryl alcohol + H2O2
-
Cyclocybe aegerita veratraldehyde + H2O
-
?
veratryl alcohol + H2O2
-
Marasmius rotula veratraldehyde + H2O
-
?

Subunits

Subunits Comment Organism
? x * 46000, SDS-PAGE Cyclocybe aegerita
? x * 32000, SDS-PAGE Marasmius rotula
? x * 44000, SDS-PAGE Coprinopsis cinerea

Synonyms

Synonyms Comment Organism
UPO
-
Coprinopsis cinerea
UPO
-
Chaetomium globosum
UPO
-
Cyclocybe aegerita
UPO
-
Marasmius rotula

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2
-
trans-stilbene at pH 7.0 and 24°C Marasmius rotula
6.6
-
4-hydroxy-trans-stilbene at pH 7.0 and 24°C Coprinopsis cinerea
9.1
-
trans-stilbene at pH 7.0 and 24°C Coprinopsis cinerea
11.7
-
4-hydroxy-trans-stilbene at pH 7.0 and 24°C Marasmius rotula
85.7
-
trans-stilbene at pH 7.0 and 24°C Cyclocybe aegerita
225
-
4-hydroxy-trans-stilbene at pH 7.0 and 24°C Cyclocybe aegerita

pI Value

Organism Comment pI Value Maximum pI Value
Marasmius rotula isoelectric focusing 5.3 5

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
250
-
4-hydroxy-trans-stilbene at pH 7.0 and 24°C Marasmius rotula
270
-
trans-stilbene at pH 7.0 and 24°C Marasmius rotula
300
-
trans-stilbene at pH 7.0 and 24°C Coprinopsis cinerea
350
-
4-hydroxy-trans-stilbene at pH 7.0 and 24°C Coprinopsis cinerea
4100
-
trans-stilbene at pH 7.0 and 24°C Cyclocybe aegerita
16000
-
4-hydroxy-trans-stilbene at pH 7.0 and 24°C Cyclocybe aegerita