Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C41 cells | Achaetomiella virescens |
expressed in Escherichia coli C41 cells | Daldinia caldariorum |
Protein Variants | Comment | Organism |
---|---|---|
F88L | the mutation strongly increases the enzyme's catalytic efficiency in oxidizing veratryl alcohol (21fold), benzyl alcohol (11fold), and naphthalene (5fold) compared to the wild type enzyme | Achaetomiella virescens |
T158F | the variant improves the oxidation only of benzyl alcohol but decreases the oxidation of veratryl alcohol and naphthalene compared with the wild type enzyme | Achaetomiella virescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) | - |
Achaetomiella virescens | |
benzyl alcohol | - |
Daldinia caldariorum | |
veratryl alcohol | - |
Daldinia caldariorum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.059 | - |
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) | wild type enzyme, at 25°C, pH not specified in the publication | Daldinia caldariorum | |
0.16 | - |
veratryl alcohol | wild type enzyme, at 25°C, pH not specified in the publication | Daldinia caldariorum | |
0.179 | - |
benzyl alcohol | wild type enzyme, at 25°C, pH not specified in the publication | Daldinia caldariorum | |
0.19 | - |
Naphthalene | mutant enzyme F88L, at 25°C, pH not specified in the publication | Achaetomiella virescens | |
0.239 | - |
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) | wild type enzyme, at 25°C, pH not specified in the publication | Achaetomiella virescens | |
0.255 | - |
Naphthalene | mutant enzyme T158F, at 25°C, pH not specified in the publication | Achaetomiella virescens | |
0.332 | - |
Naphthalene | wild type enzyme, at 25°C, pH not specified in the publication | Daldinia caldariorum | |
0.337 | - |
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) | mutant enzyme F88L, at 25°C, pH not specified in the publication | Achaetomiella virescens | |
0.337 | - |
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) | mutant enzyme T158F, at 25°C, pH not specified in the publication | Achaetomiella virescens | |
0.45 | - |
Naphthalene | wild type enzyme, at 25°C, pH not specified in the publication | Achaetomiella virescens | |
0.623 | - |
benzyl alcohol | mutant enzyme F88L, at 25°C, pH not specified in the publication | Achaetomiella virescens | |
1.15 | - |
veratryl alcohol | mutant enzyme F88L, at 25°C, pH not specified in the publication | Achaetomiella virescens | |
1.87 | - |
benzyl alcohol | mutant enzyme T158F, at 25°C, pH not specified in the publication | Achaetomiella virescens | |
2.94 | - |
veratryl alcohol | wild type enzyme, at 25°C, pH not specified in the publication | Achaetomiella virescens | |
3.97 | - |
veratryl alcohol | mutant enzyme T158F, at 25°C, pH not specified in the publication | Achaetomiella virescens | |
7.1 | - |
benzyl alcohol | wild type enzyme, at 25°C, pH not specified in the publication | Achaetomiella virescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
gel filtration | Achaetomiella virescens |
55000 | - |
gel filtration | Daldinia caldariorum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Achaetomiella virescens | - |
synonym Chaetomium virescens | - |
Daldinia caldariorum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HiTrap ion-exchange column chromatography and Superdex 75 gel filtration | Achaetomiella virescens |
HiTrap ion-exchange column chromatography and Superdex 75 gel filtration | Daldinia caldariorum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) + H2O2 | - |
Achaetomiella virescens | oxidized 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) + H2O | - |
? | |
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) + H2O2 | - |
Daldinia caldariorum | oxidized 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) + H2O | - |
? | |
4-nitrobenzaldehyde + H2O2 | - |
Achaetomiella virescens | 4-nitrobenzoic acid + H2O | - |
? | |
4-nitrobenzaldehyde + H2O2 | - |
Daldinia caldariorum | 4-nitrobenzoic acid + H2O | - |
? | |
benzyl alcohol + H2O2 | - |
Achaetomiella virescens | benzaldehyde + H2O | - |
? | |
benzyl alcohol + H2O2 | - |
Daldinia caldariorum | benzaldehyde + H2O | - |
? | |
naphthalene + H2O2 | - |
Achaetomiella virescens | ? | - |
? | |
naphthalene + H2O2 | - |
Daldinia caldariorum | ? | - |
? | |
veratryl alcohol + H2O2 | - |
Achaetomiella virescens | veratraldehyde + H2O | - |
? | |
veratryl alcohol + H2O2 | - |
Daldinia caldariorum | veratraldehyde + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 27000, SDS-PAGE | Daldinia caldariorum |
homodimer | 2 * 24000, SDS-PAGE | Achaetomiella virescens |
homodimer | 2 * 29609, calculated from amino acid sequence | Daldinia caldariorum |
homodimer | 2 * 29616, calculated from amino acid sequence | Achaetomiella virescens |
Synonyms | Comment | Organism |
---|---|---|
UPO | - |
Achaetomiella virescens |
UPO | - |
Daldinia caldariorum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
Naphthalene | mutant enzyme T158F, at 25°C, pH not specified in the publication | Achaetomiella virescens | |
1.08 | - |
Naphthalene | at 25°C, pH not specified in the publication | Achaetomiella virescens | |
1.56 | - |
veratryl alcohol | mutant enzyme T158F, at 25°C, pH not specified in the publication | Achaetomiella virescens | |
2.24 | - |
veratryl alcohol | at 25°C, pH not specified in the publication | Achaetomiella virescens | |
2.3 | - |
Naphthalene | mutant enzyme F88L, at 25°C, pH not specified in the publication | Achaetomiella virescens | |
2.39 | - |
veratryl alcohol | at 25°C, pH not specified in the publication | Daldinia caldariorum | |
6.73 | - |
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) | at 25°C, pH not specified in the publication | Daldinia caldariorum | |
11.24 | - |
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) | mutant enzyme T158F, at 25°C, pH not specified in the publication | Achaetomiella virescens | |
16.09 | - |
benzyl alcohol | at 25°C, pH not specified in the publication | Daldinia caldariorum | |
18.6 | - |
veratryl alcohol | mutant enzyme F88L, at 25°C, pH not specified in the publication | Achaetomiella virescens | |
22.1 | - |
Naphthalene | at 25°C, pH not specified in the publication | Daldinia caldariorum | |
62.9 | - |
benzyl alcohol | at 25°C, pH not specified in the publication | Achaetomiella virescens | |
63.7 | - |
benzyl alcohol | mutant enzyme F88L, at 25°C, pH not specified in the publication | Achaetomiella virescens | |
126.3 | - |
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) | mutant enzyme F88L, at 25°C, pH not specified in the publication | Achaetomiella virescens | |
153.8 | - |
benzyl alcohol | mutant enzyme T158F, at 25°C, pH not specified in the publication | Achaetomiella virescens | |
157.2 | - |
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) | at 25°C, pH not specified in the publication | Achaetomiella virescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | the enzyme contains about 0.5 mol of heme per mole of protein | Achaetomiella virescens | |
heme | the enzyme contains about 0.5 mol of heme per mole of protein | Daldinia caldariorum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
7.86 | - |
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) | at 25°C, pH not specified in the publication | Achaetomiella virescens | |
9.28 | - |
benzyl alcohol | at 25°C, pH not specified in the publication | Daldinia caldariorum | |
45.4 | - |
veratryl alcohol | at 25°C, pH not specified in the publication | Daldinia caldariorum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
veratryl alcohol | mutant enzyme T158F, at 25°C, pH not specified in the publication | Achaetomiella virescens | |
0.68 | - |
Naphthalene | mutant enzyme T158F, at 25°C, pH not specified in the publication | Achaetomiella virescens | |
0.75 | - |
veratryl alcohol | wild type enzyme, at 25°C, pH not specified in the publication | Achaetomiella virescens | |
2.42 | - |
Naphthalene | wild type enzyme, at 25°C, pH not specified in the publication | Achaetomiella virescens | |
8.9 | - |
benzyl alcohol | wild type enzyme, at 25°C, pH not specified in the publication | Achaetomiella virescens | |
12.2 | - |
Naphthalene | mutant enzyme F88L, at 25°C, pH not specified in the publication | Achaetomiella virescens | |
14.8 | - |
veratryl alcohol | wild type enzyme, at 25°C, pH not specified in the publication | Daldinia caldariorum | |
16.1 | - |
veratryl alcohol | mutant enzyme F88L, at 25°C, pH not specified in the publication | Achaetomiella virescens | |
32.1 | - |
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) | mutant enzyme T158F, at 25°C, pH not specified in the publication | Achaetomiella virescens | |
66.8 | - |
Naphthalene | wild type enzyme, at 25°C, pH not specified in the publication | Daldinia caldariorum | |
82.4 | - |
benzyl alcohol | mutant enzyme T158F, at 25°C, pH not specified in the publication | Achaetomiella virescens | |
90 | - |
benzyl alcohol | wild type enzyme, at 25°C, pH not specified in the publication | Daldinia caldariorum | |
102.1 | - |
benzyl alcohol | mutant enzyme F88L, at 25°C, pH not specified in the publication | Achaetomiella virescens | |
114.4 | - |
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) | wild type enzyme, at 25°C, pH not specified in the publication | Daldinia caldariorum | |
375 | - |
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) | mutant enzyme F88L, at 25°C, pH not specified in the publication | Achaetomiella virescens | |
656 | - |
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) | wild type enzyme, at 25°C, pH not specified in the publication | Achaetomiella virescens |