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Literature summary for 1.11.2.1 extracted from

  • Knorrscheidt, A.; Soler, J.; Huenecke, N.; Puellmann, P.; Garcia-Borras, M.; Weissenborn, M.J.
    Accessing chemo- and regioselective benzylic and aromatic oxidations by protein engineering of an unspecific peroxygenase (2021), ACS Catal., 11, 7327-7338 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae Thermothelomyces thermophilus

Protein Variants

Protein Variants Comment Organism
F59Q/L60F/S159G the mutant shows 2.6fold improved conversion efficiency with 5-nitro-1,3-benzodioxole as compared to the wild type enzyme Thermothelomyces thermophilus
F59Q/L60M/S159G/F154A the mutant shows 2.7fold improved conversion efficiency with 5-nitro-1,3-benzodioxole as compared to the wild type enzyme Thermothelomyces thermophilus
L60F the mutant shows 2.7fold improved conversion efficiency with 5-nitro-1,3-benzodioxole as compared to the wild type enzyme Thermothelomyces thermophilus
L60F/F154I the mutant shows 1.2fold improved conversion efficiency with 5-nitro-1,3-benzodioxole as compared to the wild type enzyme Thermothelomyces thermophilus
L60F/F154V the mutant shows 1.2fold improved conversion efficiency with 5-nitro-1,3-benzodioxole as compared to the wild type enzyme Thermothelomyces thermophilus
L60F/S159G/A161F the mutant shows 2.9fold improved conversion efficiency with 5-nitro-1,3-benzodioxole as compared to the wild type enzyme Thermothelomyces thermophilus
L60M the mutant shows 1.2fold improved conversion efficiency with 5-nitro-1,3-benzodioxole as compared to the wild type enzyme Thermothelomyces thermophilus
L60Q the mutant shows 1.3fold improved conversion efficiency with 5-nitro-1,3-benzodioxole as compared to the wild type enzyme Thermothelomyces thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 5-nitro-1,3-benzodioxole mutant enzyme L60F, at pH 7.0 and 25°C Thermothelomyces thermophilus
0.29
-
 5-nitro-1,3-benzodioxole mutant enzyme F59Q/L60M/S159G/F154A, at pH 7.0 and 25°C Thermothelomyces thermophilus
0.303
-
 5-nitro-1,3-benzodioxole mutant enzyme F59Q/L60F/S159G, at pH 7.0 and 25°C Thermothelomyces thermophilus
0.386
-
 5-nitro-1,3-benzodioxole wild type enzyme, at pH 7.0 and 25°C Thermothelomyces thermophilus
0.422
-
 5-nitro-1,3-benzodioxole mutant enzyme L60F/S159G, at pH 7.0 and 25°C Thermothelomyces thermophilus

Organism

Organism UniProt Comment Textmining
Thermothelomyces thermophilus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2,3,4-tetrahydronaphthalene + H2O2 reaction is performed by mutant enzyme L60F (74% ee) Thermothelomyces thermophilus (1R)-1,2,3,4-tetrahydronaphthalen-1-ol + H2O
-
 ?
1,2,3,4-tetrahydronaphthalene + H2O2 reaction is performed by mutant enzyme F59Q/L60M/S159G/F154A Thermothelomyces thermophilus 3,4-dihydronaphthalen-1(2H)-one + H2O
-
 ?
2,3-dihydro-1H-indene + H2O2 reaction is performed by mutant enzyme L60F (95% ee) Thermothelomyces thermophilus (1R)-2,3-dihydro-1H-inden-1-ol + H2O
-
 ?
2,3-dihydro-1H-indene + H2O2 reaction is performed by mutant enzyme L60F Thermothelomyces thermophilus 2,3-dihydro-1H-inden-1-one + H2O
-
 ?
2-bromonaphthalene + H2O2 reaction is performed by mutant enzyme L60F/S159G/A161F Thermothelomyces thermophilus 6-bromonaphthalene-1,4-dione + 2-bromonaphthalene-1,4-dione + H2O
-
 ?
2-methoxynaphthalene + H2O2 reaction is performed by mutant enzyme L60F/S159G/A161F Thermothelomyces thermophilus 6-methoxynaphthalene-1,4-dione + H2O
-
 ?
2-methylnaphthalene + H2O2 reaction is performed by mutant enzyme L60F/S159G/A161F Thermothelomyces thermophilus 2-methylnaphthalene-1,4-dione + H2O
-
 ?
2-methylnaphthalene + H2O2 reaction is performed by the wild type enzyme Thermothelomyces thermophilus 6-methyl-4a,8a-dihydronaphthalene-1,4-dione + H2O
-
 ?
2-methylnaphthalene + H2O2 reaction is performed by mutant enzyme L60F Thermothelomyces thermophilus naphthalen-2-ylmethanol + H2O
-
 ?
5-nitro-1,3-benzodioxole + H2O2
-
 Thermothelomyces thermophilus ?
-
 ?
naphthalene + H2O2 reaction is performed by mutant enzyme F59Q/L60F/S159G Thermothelomyces thermophilus naphthalene-1,4-dione + 2-hydroxynaphthalene-1,4-dione + H2O
-
 ?

Synonyms

Synonyms Comment Organism
UPO
-
 Thermothelomyces thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.9
-
 5-nitro-1,3-benzodioxole mutant enzyme L60F, at pH 7.0 and 25°C Thermothelomyces thermophilus
7.1
-
 5-nitro-1,3-benzodioxole wild type enzyme, at pH 7.0 and 25°C Thermothelomyces thermophilus
47
-
 5-nitro-1,3-benzodioxole mutant enzyme F59Q/L60F/S159G, at pH 7.0 and 25°C Thermothelomyces thermophilus
59.4
-
 5-nitro-1,3-benzodioxole mutant enzyme F59Q/L60M/S159G/F154A, at pH 7.0 and 25°C Thermothelomyces thermophilus
132.2
-
 5-nitro-1,3-benzodioxole mutant enzyme L60F/S159G, at pH 7.0 and 25°C Thermothelomyces thermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
19
-
 5-nitro-1,3-benzodioxole wild type enzyme, at pH 7.0 and 25°C Thermothelomyces thermophilus
27
-
 5-nitro-1,3-benzodioxole mutant enzyme L60F, at pH 7.0 and 25°C Thermothelomyces thermophilus
160
-
 5-nitro-1,3-benzodioxole mutant enzyme F59Q/L60F/S159G, at pH 7.0 and 25°C Thermothelomyces thermophilus
210
-
 5-nitro-1,3-benzodioxole mutant enzyme F59Q/L60M/S159G/F154A, at pH 7.0 and 25°C Thermothelomyces thermophilus
310
-
 5-nitro-1,3-benzodioxole mutant enzyme L60F/S159G, at pH 7.0 and 25°C Thermothelomyces thermophilus