Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using ammonium sulfate as the precipitant a total of three suitable crystal forms are obtained at different pH values (PIIpH46 with 2.0 M ammonium sulfate in 100 mM TrisHCl pH 8.5, PIIpH56 with 2.4 M ammonium sulfate in 100 mM sodium citrate pH 5.6, and PIIpH85 with 2.0 M ammonium sulfate in 200 mM sodium acetate pH 4.6) | Cyclocybe aegerita |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
SDS-PAGE | Cyclocybe aegerita |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cyclocybe aegerita | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | highly glycosylated peroxygenase | Cyclocybe aegerita |
Purification (Comment) | Organism |
---|---|
SP Sepharose column chromatography, Mono Q column chromatography, and Mono S column chromatography | Cyclocybe aegerita |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
veratryl alcohol + H2O2 + H+ | - |
Cyclocybe aegerita | veratraldehyde + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
45 kDa peroxygenase/peroxidase | - |
Cyclocybe aegerita |
APO | - |
Cyclocybe aegerita |
aromatic peroxygenase | - |
Cyclocybe aegerita |
PII | - |
Cyclocybe aegerita |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Cyclocybe aegerita |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cyclocybe aegerita | the enzyme has two pI-values of 5.6 and 5.2, isoelectric focusing | - |
5.2 |
Cyclocybe aegerita | the enzyme has two pI-values of 5.6 and 5.2, isoelectric focusing | - |
5.6 |