Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Zobellia galactanivorans |
Crystallization (Comment) | Organism |
---|---|
to 1.8 A resolution, revealing a monomeric structure mainly folded into alpha-helices. The vanadate binding site is strictly conserved with the fungal VCPO active site. Specific amino acids and the associated hydrogen bonding network around the vanadate center are essential for the catalytic properties and also the iodide specificity | Zobellia galactanivorans |
Protein Variants | Comment | Organism |
---|---|---|
C320S | 34% of wild-type activity | Zobellia galactanivorans |
D322L | 31% of wild-type activity | Zobellia galactanivorans |
D322Y | 0.3% of wild-type activity | Zobellia galactanivorans |
F353H | 225% of wild-type activity | Zobellia galactanivorans |
H360A | 6% of wild-type activity | Zobellia galactanivorans |
H360S | 0.4% of wild-type activity | Zobellia galactanivorans |
R410A | 0.1% of wild-type activity | Zobellia galactanivorans |
S358A | 128% of wild-type activity | Zobellia galactanivorans |
W321R | 51% of wild-type activity | Zobellia galactanivorans |
Y263A | 89% of wild-type activity | Zobellia galactanivorans |
Y263F | 38% of wild-type activity | Zobellia galactanivorans |
Y263S | 98% of wild-type activity | Zobellia galactanivorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
thymol blue | mutant H360A, pH 7.2, 20°C | Zobellia galactanivorans | |
0.03 | - |
thymol blue | mutant D322Y, pH 7.2, 20°C | Zobellia galactanivorans | |
0.05 | - |
thymol blue | mutant H360S, pH 7.2, 20°C | Zobellia galactanivorans | |
0.19 | - |
thymol blue | mutant S358A, pH 7.2, 20°C | Zobellia galactanivorans | |
0.22 | - |
thymol blue | wild-type, pH 7.2, 20°C | Zobellia galactanivorans | |
0.39 | - |
thymol blue | mutant W321R, pH 7.2, 20°C | Zobellia galactanivorans | |
0.44 | - |
thymol blue | mutant Y263A, pH 7.2, 20°C | Zobellia galactanivorans | |
0.79 | - |
thymol blue | mutant Y263F, pH 7.2, 20°C | Zobellia galactanivorans | |
0.91 | - |
thymol blue | mutant C320S, pH 7.2, 20°C | Zobellia galactanivorans | |
1.38 | - |
thymol blue | mutant D322L, pH 7.2, 20°C | Zobellia galactanivorans | |
1.58 | - |
thymol blue | mutant Y263S, pH 7.2, 20°C | Zobellia galactanivorans | |
2.63 | - |
thymol blue | mutant F353H, pH 7.2, 20°C | Zobellia galactanivorans | |
3.45 | - |
thymol blue | mutant R410A, pH 7.2, 20°C | Zobellia galactanivorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zobellia galactanivorans | G0LAH5 | - |
- |
Zobellia galactanivorans DSM 12802 | G0LAH5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme shows strict specificity for iodide oxidation | Zobellia galactanivorans | ? | - |
? | |
additional information | enzyme shows strict specificity for iodide oxidation | Zobellia galactanivorans DSM 12802 | ? | - |
? | |
thymol blue + 2 I- + 2 H+ + H2O2 | - |
Zobellia galactanivorans | diiodothymol blue + 2 H2O | - |
? | |
thymol blue + 2 I- + 2 H+ + H2O2 | - |
Zobellia galactanivorans DSM 12802 | diiodothymol blue + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
zobellia_1262 | - |
Zobellia galactanivorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
thymol blue | mutant D322Y, pH 7.2, 20°C | Zobellia galactanivorans | |
0.002 | - |
thymol blue | mutant H360S, pH 7.2, 20°C | Zobellia galactanivorans | |
0.01 | - |
thymol blue | mutant H360A, pH 7.2, 20°C | Zobellia galactanivorans | |
0.03 | - |
thymol blue | mutant R410A, pH 7.2, 20°C | Zobellia galactanivorans | |
1.78 | - |
thymol blue | mutant W321R, pH 7.2, 20°C | Zobellia galactanivorans | |
1.98 | - |
thymol blue | wild-type, pH 7.2, 20°C | Zobellia galactanivorans | |
2.19 | - |
thymol blue | mutant S358A, pH 7.2, 20°C | Zobellia galactanivorans | |
2.67 | - |
thymol blue | mutant Y263F, pH 7.2, 20°C | Zobellia galactanivorans | |
2.76 | - |
thymol blue | mutant C320S, pH 7.2, 20°C | Zobellia galactanivorans | |
3.53 | - |
thymol blue | mutant Y263A, pH 7.2, 20°C | Zobellia galactanivorans | |
3.86 | - |
thymol blue | mutant D322L, pH 7.2, 20°C | Zobellia galactanivorans | |
13.97 | - |
thymol blue | mutant Y263S, pH 7.2, 20°C | Zobellia galactanivorans | |
53.27 | - |
thymol blue | mutant F353H, pH 7.2, 20°C | Zobellia galactanivorans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
thymol blue | mutant R410A, pH 7.2, 20°C | Zobellia galactanivorans | |
0.02 | - |
thymol blue | mutant D322Y, pH 7.2, 20°C | Zobellia galactanivorans | |
0.04 | - |
thymol blue | mutant H360S, pH 7.2, 20°C | Zobellia galactanivorans | |
0.5 | - |
thymol blue | mutant H360A, pH 7.2, 20°C | Zobellia galactanivorans | |
2.8 | - |
thymol blue | mutant D322L, pH 7.2, 20°C | Zobellia galactanivorans | |
3.03 | - |
thymol blue | mutant C320S, pH 7.2, 20°C | Zobellia galactanivorans | |
3.38 | - |
thymol blue | mutant Y263F, pH 7.2, 20°C | Zobellia galactanivorans | |
4.56 | - |
thymol blue | mutant W321R, pH 7.2, 20°C | Zobellia galactanivorans | |
8.02 | - |
thymol blue | mutant Y263A, pH 7.2, 20°C | Zobellia galactanivorans | |
8.84 | - |
thymol blue | mutant Y263S, pH 7.2, 20°C | Zobellia galactanivorans | |
9 | - |
thymol blue | wild-type, pH 7.2, 20°C | Zobellia galactanivorans | |
11.53 | - |
thymol blue | mutant S358A, pH 7.2, 20°C | Zobellia galactanivorans | |
20.25 | - |
thymol blue | mutant F353H, pH 7.2, 20°C | Zobellia galactanivorans |