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Literature summary for 1.11.1.9 extracted from

  • Song, J.; Yu, Y.; Xing, R.; Guo, X.; Liu, D.; Wei, J.; Song, H.
    Unglycosylated recombinant human glutathione peroxidase 3 mutant from Escherichia coli is active as a monomer (2014), Sci. Rep., 4, 6698 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular modeling of mutant with all Cys residues changed to Ser Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information expression of a recombinant human Gpx3 mutant with all Cys residues changed to Ser from a Cys auxotrophic strain of E. coli. Although lacking post-translational modification, the mutant still retains the ability to reduce H2O2 and 1-palmitoyl-2-(13-hydroperoxy-cis-9, trans-11-octadecadienoyl)-L-3-phosphatidylcholine. Contrary to wild-type, the mutant exists as a monomer in solution Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P22352
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.5
-
mutant with all Cys residues changed to Ser, substrate 1-palmitoyl-2-(13-hydroperoxy-cis-9, trans-11-octadecadienoyl)-L-3-phosphatidylcholine, pH 7.4, 37°C Homo sapiens
25
-
mutant with all Cys residues changed to Ser, substrate H2O2, pH 7.4, 37°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-palmitoyl-2-(13-hydroperoxy-cis-9, trans-11-octadecadienoyl)-L-3-phosphatidylcholine + 2 GSH
-
Homo sapiens 1-palmitoyl-2-(13-hydroxy-cis-9, trans-11-octadecadienoyl)-L-3-phosphatidylcholine + GSSG + H2O
-
?
2 glutathione + H2O2
-
Homo sapiens glutathione disulfide + 2 H2O
-
?

Subunits

Subunits Comment Organism
monomer 1 * 26000, SDS-PAGE, recombinant mutant with all Cys residues changed to Ser Homo sapiens

Synonyms

Synonyms Comment Organism
GPX3
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
mutant with all Cys residues changed to Ser Homo sapiens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
55
-
almost completely inactive Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.9
-
mutant with all Cys residues changed to Ser Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.4 8.9
-
Homo sapiens
9.8
-
93% loss of activity Homo sapiens