Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
molecular modeling of mutant with all Cys residues changed to Ser | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression of a recombinant human Gpx3 mutant with all Cys residues changed to Ser from a Cys auxotrophic strain of E. coli. Although lacking post-translational modification, the mutant still retains the ability to reduce H2O2 and 1-palmitoyl-2-(13-hydroperoxy-cis-9, trans-11-octadecadienoyl)-L-3-phosphatidylcholine. Contrary to wild-type, the mutant exists as a monomer in solution | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P22352 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.5 | - |
mutant with all Cys residues changed to Ser, substrate 1-palmitoyl-2-(13-hydroperoxy-cis-9, trans-11-octadecadienoyl)-L-3-phosphatidylcholine, pH 7.4, 37°C | Homo sapiens |
25 | - |
mutant with all Cys residues changed to Ser, substrate H2O2, pH 7.4, 37°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-palmitoyl-2-(13-hydroperoxy-cis-9, trans-11-octadecadienoyl)-L-3-phosphatidylcholine + 2 GSH | - |
Homo sapiens | 1-palmitoyl-2-(13-hydroxy-cis-9, trans-11-octadecadienoyl)-L-3-phosphatidylcholine + GSSG + H2O | - |
? | |
2 glutathione + H2O2 | - |
Homo sapiens | glutathione disulfide + 2 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 26000, SDS-PAGE, recombinant mutant with all Cys residues changed to Ser | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
GPX3 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
mutant with all Cys residues changed to Ser | Homo sapiens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
almost completely inactive | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.9 | - |
mutant with all Cys residues changed to Ser | Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.4 | 8.9 | - |
Homo sapiens |
9.8 | - |
93% loss of activity | Homo sapiens |