Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C2S/C78S/C115S/C156S/C202S | about 10fold increase in activity compared to wild-type. Mutant displays a typical ping-pong mechanism | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
H2O2 | mutant C2S/C78S/C115S/C156S/C202S, presence of 3 mM GSH, pH 7.4, 37°C | Homo sapiens | |
0.022 | - |
glutathione | mutant C2S/C78S/C115S/C156S/C202S, presence of 0.03 mM H2O2, pH 7.4, 37°C | Homo sapiens | |
0.31 | - |
glutathione | mutant C2S/C78S/C115S/C156S/C202S, presence of 0.10 mM H2O2, pH 7.4, 37°C | Homo sapiens | |
0.31 | - |
H2O2 | mutant C2S/C78S/C115S/C156S/C202S, presence of 10 mM GSH, pH 7.4, 37°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
selenium | selenium-dependent enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P07203 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Hep-G2 cell | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.52 | - |
wild-type, pH 7.4, 37°C | Homo sapiens |
5.28 | - |
mutant C2S/C78S/C115S/C156S/C202S, pH 7.4, 37°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 glutathione + H2O2 | - |
Homo sapiens | glutathione disulfide + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GPX1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
mutant C2S/C78S/C115S/C156S/C202S | Homo sapiens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
75% of maximum activity, mutant C2S/C78S/C115S/C156S/C202S | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.7 | - |
H2O2 | mutant C2S/C78S/C115S/C156S/C202S, presence of 3 mM GSH, pH 7.4, 37°C | Homo sapiens | |
4.8 | - |
glutathione | mutant C2S/C78S/C115S/C156S/C202S, presence of 0.03 mM H2O2, pH 7.4, 37°C | Homo sapiens | |
683 | - |
glutathione | mutant C2S/C78S/C115S/C156S/C202S, presence of 0.10 mM H2O2, pH 7.4, 37°C | Homo sapiens | |
727.8 | - |
H2O2 | mutant C2S/C78S/C115S/C156S/C202S, presence of 10 mM GSH, pH 7.4, 37°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
wild-type | Homo sapiens |
9 | - |
mutant C2S/C78S/C115S/C156S/C202S | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
26% of maximum activity, mutant C2S/C78S/C115S/C156S/C202S | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
216.7 | - |
glutathione | mutant C2S/C78S/C115S/C156S/C202S, presence of 0.03 mM H2O2, pH 7.4, 37°C | Homo sapiens | |
216.7 | - |
glutathione | mutant C2S/C78S/C115S/C156S/C202S, presence of 0.10 mM H2O2, pH 7.4, 37°C | Homo sapiens | |
231.7 | - |
H2O2 | mutant C2S/C78S/C115S/C156S/C202S, presence of 10 mM GSH, pH 7.4, 37°C | Homo sapiens | |
231.7 | - |
H2O2 | mutant C2S/C78S/C115S/C156S/C202S, presence of 3 mM GSH, pH 7.4, 37°C | Homo sapiens |