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Literature summary for 1.11.1.9 extracted from

  • Yang, W.H.; Park, S.Y.; Ji, S.; Kang, J.G.; Kim, J.-E.; Song, H.; Mook-Jung, I.; Choe, K.-M.; Cho, J.W.
    O-GlcNAcylation regulates hyperglycemia-induced GPX1 activation (2010), Biochem. Biophys. Res. Commun., 391, 756-761.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
hyperglycemia increased GPX1 activity in rat VSM cell. When cells are exposed to high glucose (25 mM) for 48 h, GPX1 activity is 2.5fold greater than in cells grown in normal glucose (5 mM), while the GPX1 expression levels are the same Rattus norvegicus
additional information increasing the cellular O-GlcNAcylation level enhances GPX1 activity Rattus norvegicus
additional information injection of the O-GlcNAcase inhibitor 1,2-dideoxy-2'-propyl-alpha-D-glucopyranoso-[2,1-d]-DELTA2'-thiazoline increases GPX1 activity in the mouse liver Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in HEK-293T cells Rattus norvegicus
expression constructs encoding full-length (1-201) and C-terminal deletion (1-100) versions of human GPX1 (Sec47Cys) for overexpression in HEK-293 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information full-length (1-201) and C-terminal deletion (1-100) versions of human GPX1 (Sec47Cys), full-length FLAG-GPX1 is modified with O-GlcNAc, while the truncated FLAG-GPX1 is not, despite the fact that truncated GPX1 is still able to bind OGT. O-GlcNAcylation occurs on the C-terminal half of GPX1 Homo sapiens
additional information GPX1 modified with O-GlcNAc on its C-terminus, cell lysate of VSMCs treated with the O-GlcNAcase inhibitor PUGNAc Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
selenium selenium-containing enzyme Mus musculus
selenium selenium-containing enzyme Homo sapiens
selenium selenium-containing enzyme Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
B6C3F1/J mice, mating C57BL/6J female mice to C3H/HeJ male mice
-
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Mus musculus
-
muscle vascular smooth muscles cell Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2O2 + GSH instead of tert-butyl hydroperoxide Mus musculus H2O + GSSG
-
?
tert-butyl hydroperoxide + 2 GSH
-
Homo sapiens tert-butyl alcohol + GSSG + H2O
-
?
tert-butyl hydroperoxide + 2 GSH
-
Rattus norvegicus tert-butyl alcohol + GSSG + H2O
-
?

Synonyms

Synonyms Comment Organism
glutathione peroxidase 1
-
Mus musculus
glutathione peroxidase 1
-
Homo sapiens
glutathione peroxidase 1
-
Rattus norvegicus
GPX1
-
Mus musculus
GPX1
-
Homo sapiens
GPX1
-
Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Mus musculus
25
-
assay at Homo sapiens
25
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Mus musculus
8
-
assay at Homo sapiens
8
-
assay at Rattus norvegicus

General Information

General Information Comment Organism
physiological function anti-oxidant enzyme, essential for cell survival during oxidative stress Mus musculus
physiological function anti-oxidant enzyme, essential for cell survival during oxidative stress Homo sapiens
physiological function anti-oxidant enzyme, essential for cell survival during oxidative stress Rattus norvegicus