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Literature summary for 1.11.1.9 extracted from

  • Liu, L.; Mao, S.Z.; Liu, X.M.; Huang, X.; Xu, J.Y.; Liu, J.Q.; Luo, G.M.; Shen, J.C.
    Functional mimicry of the active site of glutathione peroxidase by glutathione imprinted selenium-containing protein (2008), Biomacromolecules, 9, 363-368.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
GSH apparent value, imprinted GSH-selenosubtilisin in the presence of 0.5 mM H2O2, in 50 mM PIPES buffer, pH 7.0 at 37°C Homo sapiens
0.3
-
GSH apparent value, imprinted GSH-selenosubtilisin in the presence of 1 mM H2O2, in 50 mM PIPES buffer, pH 7.0 at 37°C Homo sapiens
0.4
-
GSH apparent value, imprinted GSH-selenosubtilisin in the presence of 2 mM H2O2, in 50 mM PIPES buffer, pH 7.0 at 37°C Homo sapiens
0.45
-
H2O2 apparent value, imprinted GSH-selenosubtilisin in the presence of 0.5 mM GSH, in 50 mM PIPES buffer, pH 7.0 at 37°C Homo sapiens
0.62
-
H2O2 apparent value, imprinted GSH-selenosubtilisin in the presence of 1 mM GSH, in 50 mM PIPES buffer, pH 7.0 at 37°C Homo sapiens
0.77
-
H2O2 apparent value, imprinted GSH-selenosubtilisin in the presence of 2 mM GSH, in 50 mM PIPES buffer, pH 7.0 at 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2O2 + GSH
-
Homo sapiens H2O + GSSG
-
?
tert-butylhydroperoxide + 3-carboxy-4-nitrobenzenethiol
-
Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
GPX
-
Homo sapiens
selenosubtilisin artificial enzyme that imitates the active site of antioxidant selenoenzyme glutathione peroxidase, the imprinted selenium-containing protein exhibits remarkable GPx activity Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10.55
-
H2O2 apparent value, imprinted GSH-selenosubtilisin in the presence of 0.5 mM GSH, in 50 mM PIPES buffer, pH 7.0 at 37°C Homo sapiens
14.48
-
GSH apparent value, imprinted GSH-selenosubtilisin in the presence of 0.5 mM H2O2, in 50 mM PIPES buffer, pH 7.0 at 37°C Homo sapiens
16.02
-
H2O2 apparent value, imprinted GSH-selenosubtilisin in the presence of 1 mM GSH, in 50 mM PIPES buffer, pH 7.0 at 37°C Homo sapiens
19.5
-
GSH apparent value, imprinted GSH-selenosubtilisin in the presence of 1 mM H2O2, in 50 mM PIPES buffer, pH 7.0 at 37°C Homo sapiens
20.83
-
H2O2 apparent value, imprinted GSH-selenosubtilisin in the presence of 2 mM GSH, in 50 mM PIPES buffer, pH 7.0 at 37°C Homo sapiens
24.5
-
GSH apparent value, imprinted GSH-selenosubtilisin in the presence of 2 mM H2O2, in 50 mM PIPES buffer, pH 7.0 at 37°C Homo sapiens