KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
I- | in phosphate buffer (100 mM, pH 8.0), at 20°C | Ascophyllum nodosum | |
22 | - |
Br- | - |
Ascophyllum nodosum | |
22 | - |
Br- | in phosphate buffer (100 mM, pH 8.0), at 20°C | Ascophyllum nodosum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ascophyllum nodosum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Ascophyllum nodosum |
ammonium sulfate precipitation and phenyl-Sepharose column chromatography | Ascophyllum nodosum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
thallus | - |
Ascophyllum nodosum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
an accurate and sensitive colorimetric assay for the titration of haloperoxidase activities is developed. The method is based on the specific increase in absorbance at 620 nm that occurs on the halogenation of thymol blue. The quantitative determination of both iodo- and bromoperoxidase activities with the same assay is now possible | Ascophyllum nodosum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Br- + H2O2 + thymol blue | ordered two-substrate mechanism | Ascophyllum nodosum | ? | - |
? | |
Br- + H2O2 + thymol blue | - |
Ascophyllum nodosum | dibromothymolsulfonphthalein + ? | - |
? | |
I- + H2O2 + thymol blue | - |
Ascophyllum nodosum | diiodothymolsulfonphthalein + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
bromoperoxidase | - |
Ascophyllum nodosum |
vanadium bromoperoxidase | - |
Ascophyllum nodosum |
vBPO | - |
Ascophyllum nodosum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
71 | - |
Br- | - |
Ascophyllum nodosum | |
71 | - |
Br- | in phosphate buffer (100 mM, pH 8.0), at 20°C | Ascophyllum nodosum | |
75 | - |
I- | in phosphate buffer (100 mM, pH 8.0), at 20°C | Ascophyllum nodosum |