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Literature summary for 1.11.1.8 extracted from

  • Verhaeghe, E.; Buisson, D.; Zekri, E.; Leblanc, C.; Potin, P.; Ambroise, Y.
    A colorimetric assay for steady-state analyses of iodo- and bromoperoxidase activities (2008), Anal. Biochem., 379, 60-65.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
I- in phosphate buffer (100 mM, pH 8.0), at 20°C Ascophyllum nodosum
22
-
Br-
-
Ascophyllum nodosum
22
-
Br- in phosphate buffer (100 mM, pH 8.0), at 20°C Ascophyllum nodosum

Organism

Organism UniProt Comment Textmining
Ascophyllum nodosum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Ascophyllum nodosum
ammonium sulfate precipitation and phenyl-Sepharose column chromatography Ascophyllum nodosum

Source Tissue

Source Tissue Comment Organism Textmining
thallus
-
Ascophyllum nodosum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
an accurate and sensitive colorimetric assay for the titration of haloperoxidase activities is developed. The method is based on the specific increase in absorbance at 620 nm that occurs on the halogenation of thymol blue. The quantitative determination of both iodo- and bromoperoxidase activities with the same assay is now possible Ascophyllum nodosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Br- + H2O2 + thymol blue ordered two-substrate mechanism Ascophyllum nodosum ?
-
?
Br- + H2O2 + thymol blue
-
Ascophyllum nodosum dibromothymolsulfonphthalein + ?
-
?
I- + H2O2 + thymol blue
-
Ascophyllum nodosum diiodothymolsulfonphthalein + ?
-
?

Synonyms

Synonyms Comment Organism
bromoperoxidase
-
Ascophyllum nodosum
vanadium bromoperoxidase
-
Ascophyllum nodosum
vBPO
-
Ascophyllum nodosum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
71
-
Br-
-
Ascophyllum nodosum
71
-
Br- in phosphate buffer (100 mM, pH 8.0), at 20°C Ascophyllum nodosum
75
-
I- in phosphate buffer (100 mM, pH 8.0), at 20°C Ascophyllum nodosum