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Literature summary for 1.11.1.8 extracted from

  • Tsyganova, O.V.; Kiseleva, E.P.; Vashkevich, I.I.; Pryadko, A.G.; Sviridov, O.V.
    Characteristics of immunoaffinity chromatography and a new method for isolation of human thyroid peroxidase from subcellular fractions of thyroid gland (2006), Appl. Biochem. Microbiol., 42, 210-219.
No PubMed abstract available

Application

Application Comment Organism
analysis development of an ELISA system for quantitative determination of TPO in biological fluids, based on the use of an analytical pair of monoclonal antibodies, one of which is immobilized on solid phase, the other being present in solution. The use of this system made it possible to determine the biochemical characteristics of immunoaffinity chromatography of the enzyme and propose a new method for obtaining a highly purified preparation of its protein Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-
microsome
-
Homo sapiens
-
-
mitochondrion
-
Homo sapiens 5739
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
thyroid gland of patients with diverse thyroid diseases Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
development of an ELISA system for quantitative determination of thyroid peroxidase in biological fluids, based on the use of an analytical pair of monoclonal antibodies, one of which is immobilized on solid phase, the other being present in solution. The use of this system made it possible to determine the biochemical characteristics of immunoaffinity chromatography of the enzyme and propose a new method for obtaining a highly purified preparation of its protein Homo sapiens

Storage Stability

Storage Stability Organism
-18°C or -4°C, enzyme retains its immunoreactivity Homo sapiens
-18°C, 4 months, ability of the protein to interact with autoantibodies is decreased by 20% Homo sapiens

Synonyms

Synonyms Comment Organism
thyroid peroxidase
-
Homo sapiens
TPO
-
Homo sapiens