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Literature summary for 1.11.1.7 extracted from

  • Sarvandi-Dehghanpoor, E.; Riahi-Madvar, A.; Lotfi, S.; Torkzadeh-Mahani, M.
    Improvement of kinetic properties and thermostability of recombinant Lepidium draba peroxidase (LDP) upon exposed to osmolytes (2018), Int. J. Biol. Macromol., 119, 1036-1041 .
    View publication on PubMed
Search for more literature for 1.11.1.7

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Lepidium draba

General Stability

General Stability Organism
structural changes of the enzyme upon exposed to the osmolytes glycine and D-sorbitol, leads to the improvement of stability. Lepidium draba

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 3,3',5,5'-tetramethylbenzidine pH 6.5, 30°C, 600 mM sorbotol Lepidium draba
0.11
-
 3,3',5,5'-tetramethylbenzidine pH 6.5, 30°C, 300 mM glycine Lepidium draba
0.2
-
 3,3',5,5'-tetramethylbenzidine pH 6.5, 30°C Lepidium draba
4.5
-
 H2O2 pH 6.5, 30°C, 600 mM sorbitol Lepidium draba
5.1
-
 H2O2 pH 6.5, 30°C, 300 mM glycine Lepidium draba
8.74
-
 H2O2 pH 6.5, 30°C Lepidium draba

Organism

Organism UniProt Comment Textmining
Lepidium draba A0A076L8M7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lepidium draba

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,3',5,5'-tetramethylbenzidine + H2O2
-
 Lepidium draba ? + H2O
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 optimum temperature without osmolyte Lepidium draba
53
-
 optimum temperature in the presence of glycine (300 mM) Lepidium draba
60
-
 optimum temperature in the presence of sorbitol (600 mM) Lepidium draba