Application | Comment | Organism |
---|---|---|
synthesis | protein engineering to obtain an active and stable HRP variant with reduced surface glycosylation during production in Pichia pastoris. Mutant N13D/N57S/N255D/N268D produced in the Pichia pastoris benchmark strain shows considerable catalytic activity and thermal stability and is less glycosylated | Armoracia rusticana |
Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Armoracia rusticana |
Protein Variants | Comment | Organism |
---|---|---|
N13D/N57S/N255D/N268D | mutations introduced to reduce glycosylation during production in Pichia pastoris | Armoracia rusticana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
H2O2 | wild-type, pH 6.5, 30°C | Armoracia rusticana | |
0.015 | - |
H2O2 | mutant N13D/N57S/N255D/N268D, pH 6.5, 30°C | Armoracia rusticana | |
0.99 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant N13D/N57S/N255D/N268D, pH 6.5, 30°C | Armoracia rusticana | |
1.5 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) | wild-type, pH 6.5, 30°C | Armoracia rusticana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Armoracia rusticana | P00433 | isoform C1A | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Armoracia rusticana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Armoracia rusticana | ? + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 65000, SDS-PAGE, wild-type. x * 50000, SDS-PAGE, glycosylation mutant N13D/N57S/N255D/N268D | Armoracia rusticana |
Synonyms | Comment | Organism |
---|---|---|
HRP C1A | - |
Armoracia rusticana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
half-life of wild-type 31.5 min, half-life of mutant N13D/N57S/N255D/N268D 173 min | Armoracia rusticana |