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Literature summary for 1.11.1.7 extracted from
- Engineering a horseradish peroxidase C stable to radical attacks by mutating multiple radical coupling sites (2015), Biotechnol. Bioeng., 112, 668-676 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F130A/F142A/ F143A/F179A | isoform HRP C, mutant shows marginal improvement in stability | Armoracia rusticana |
| F142A | isoform HRP C, mutation in a residue vulnerable to modification by phenoxyl radicals, mutant still exhibits rapid radical inactivation | Armoracia rusticana |
| F143A | isoform HRP C, mutation in a residue vulnerable to modification by phenoxyl radicals, mutant still exhibits rapid radical inactivation | Armoracia rusticana |
| F179A | isoform HRP C, mutation in a residue vulnerable to modification by phenoxyl radicals, mutant still exhibits rapid radical inactivation | Armoracia rusticana |
| F68A | isoform HRP C, mutation in a residue vulnerable to modification by phenoxyl radicals, mutant still exhibits rapid radical inactivation | Armoracia rusticana |
| F68A/F142A/F143A/F179A | isoform HRP C, dramatic enhancement of radical stability by retaining 41% of its initial activity in conditions when wild-type is completely inactivated | Armoracia rusticana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phenoxy radical | phenylalanine residues are vulnerable to modification by phenoxyl radicals. Radical coupling does not change the secondary structure or the active site of HRP isoform C | Armoracia rusticana |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.966 | - |
guaiacol | mutant F130A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
| 0.966 | - |
guaiacol | wild-type isoform C, pH 5, temperature not specified in the publication | Armoracia rusticana | |
| 1.06 | - |
guaiacol | mutant F68A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
| 1.72 | - |
guaiacol | mutant F143A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
| 1.82 | - |
guaiacol | mutant F179A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
| 2.04 | - |
guaiacol | mutant F142A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
| 2.16 | - |
guaiacol | mutant F130A/F142A/ F143A/F179A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
| 2.69 | - |
guaiacol | mutant F68A/F142A/ F143A/F179A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Armoracia rusticana | P80679 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Armoracia rusticana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 guaiacol + H2O2 | - |
Armoracia rusticana | 3,3'-dimethoxy-4,4'-biphenylquinone + H2O | - |
? | |
| 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) + H2O2 | - |
Armoracia rusticana | ? + H2O | - |
? | |
| phenol + H2O2 | - |
Armoracia rusticana | ? + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HRP A2 | - |
Armoracia rusticana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.952 | - |
guaiacol | mutant F68A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
| 1.24 | - |
guaiacol | mutant F142A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
| 1.41 | - |
guaiacol | mutant F130A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
| 1.44 | - |
guaiacol | wild-type isoform C, pH 5, temperature not specified in the publication | Armoracia rusticana | |
| 1.55 | - |
guaiacol | mutant F143A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
| 1.83 | - |
guaiacol | mutant F179A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
| 2.09 | - |
guaiacol | mutant F130A/F142A/ F143A/F179A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
| 2.3 | - |
guaiacol | mutant F68A/F142A/ F143A/F179A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.61 | - |
guaiacol | mutant F142A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
| 0.86 | - |
guaiacol | mutant F68A/F142A/ F143A/F179A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
| 0.89 | - |
guaiacol | mutant F143A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
| 0.89 | - |
guaiacol | mutant F68A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
| 1.01 | - |
guaiacol | mutant F179A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
| 1.07 | - |
guaiacol | mutant F130A/F142A/ F143A/F179A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
| 1.46 | - |
guaiacol | mutant F130A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
| 1.49 | - |
guaiacol | wild-type isoform C, pH 5, temperature not specified in the publication | Armoracia rusticana | |
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