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Literature summary for 1.11.1.7 extracted from

  • Patel, A.K.; Singh, V.K.; Moir, A.J.; Jagannadham, M.V.
    Biochemical and spectroscopic characterization of morning glory peroxidase from an invasive and hallucinogenic plant weed Ipomoea carnea (2008), J. Agric. Food Chem., 56, 9236-9245.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
K2Cr2O7 108.8% relative activity at 10 mM Ipomoea carnea

Inhibitors

Inhibitors Comment Organism Structure
acetonitrile 88.0% residual activity at 60% (v/v) Ipomoea carnea
beta-mercaptoethanol
-
Ipomoea carnea
butanol 81.0% residual activity at 30% (v/v) Ipomoea carnea
Ca2+ 61.8% relative activity at 10 mM Ipomoea carnea
Co2+ 51.9% relative activity at 10 mM Ipomoea carnea
Cs+ 86.3% relative activity at 10 mM Ipomoea carnea
Cu2+ 80.8% relative activity at 10 mM Ipomoea carnea
dioxane 82.0% residual activity at 50% (v/v) Ipomoea carnea
DMSO 76.0% residual activity at 30% (v/v) Ipomoea carnea
EDTA 87.3% relative activity at 10 mM Ipomoea carnea
ethanol 82.0% residual activity at 65% (v/v) Ipomoea carnea
glutathione
-
Ipomoea carnea
guanidine hydrochloride 84.2% residual activity at 3 mM Ipomoea carnea
guanidine isothiocyanate 82.4% residual activity at 1.5 mM Ipomoea carnea
Hg2+ complete inhibition at 10 mM Ipomoea carnea
hydroxylamine
-
Ipomoea carnea
Isopropanol 80.0% residual activity at 45% (v/v) Ipomoea carnea
K+ 92.6% relative activity at 10 mM Ipomoea carnea
L-cysteine
-
Ipomoea carnea
methanol 85.0% residual activity at 70% (v/v) Ipomoea carnea
Mg2+ 83.5% relative activity at 10 mM Ipomoea carnea
Mn2+ 93.2% relative activity at 10 mM Ipomoea carnea
additional information inhibition is not observed with ethylmaleinimide and urea Ipomoea carnea
Na+ 94.8% relative activity at 10 mM Ipomoea carnea
Ni2+ 90.6% relative activity at 10 mM Ipomoea carnea
Pb2+ 91.8% relative activity at 10 mM Ipomoea carnea
SDS 81.7% residual activity at 10 mM Ipomoea carnea
Sodium azide
-
Ipomoea carnea
Zn2+ 95.0% relative activity at 10 mM Ipomoea carnea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
4-aminoantipyrine at pH 7.0 Ipomoea carnea
0.29
-
pyrogallol at pH 5.5 Ipomoea carnea
0.5
-
o-Dianisidine at pH 8.0 Ipomoea carnea
1.63
-
pyrogallol at pH 6.0 Ipomoea carnea
2.02
-
o-phenylenediamine at pH 5.5 Ipomoea carnea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000 45000 the purified enzyme migrates on SDS-PAGE as a single band with an apparent molecular mass of 40000-45000 Da Ipomoea carnea
42060
-
MALDI-TOF mass spectrometry Ipomoea carnea

Organism

Organism UniProt Comment Textmining
Ipomoea carnea
-
subspecies Ipomoea carnea fistulosa, morning glory
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Ipomoea carnea

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, ether-Toyopearl column chromatography, and Sephacryl S-200 gel filtration Ipomoea carnea

Source Tissue

Source Tissue Comment Organism Textmining
latex
-
Ipomoea carnea
-
stem
-
Ipomoea carnea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-aminoantipyrine + H2O2
-
Ipomoea carnea ?
-
?
guaiacol + H2O2
-
Ipomoea carnea tetraguaiacol + H2O
-
?
o-dianisidine + H2O2
-
Ipomoea carnea ?
-
?
o-phenylenediamine + H2O2
-
Ipomoea carnea ?
-
?
pyrogallol + H2O2
-
Ipomoea carnea purpurogallin + H2O
-
?

Synonyms

Synonyms Comment Organism
donor: H2O2 oxidoreductase
-
Ipomoea carnea
MGP
-
Ipomoea carnea

Cofactor

Cofactor Comment Organism Structure
heme
-
Ipomoea carnea

pI Value

Organism Comment pI Value Maximum pI Value
Ipomoea carnea isoelectric focusing
-
4.3