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Literature summary for 1.11.1.7 extracted from

  • Ogola, H.J.; Kamiike, T.; Hashimoto, N.; Ashida, H.; Ishikawa, T.; Shibata, H.; Sawa, Y.
    Molecular characterization of a novel DyP-type peroxidase from the cyanobacterium Anabaena sp. PCC 7120 (2009), Appl. Environ. Microbiol., 75, 7509-7518.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.7

Activating Compound

Activating Compound Comment Organism Structure
syringaldehyde the decolorization activity of AnaPX toward azo dyes is significantly enhanced 2-50fold in the presence of syringaldehyde Anabaena sp.

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Anabaena sp.

Inhibitors

Inhibitors Comment Organism Structure
diethyl dicarbonate less than 50% inhibition at 50 mM Anabaena sp.
Fe2+ 98.8% inhibition at 5 mM Anabaena sp.
KCN very low sensitivity, causes only 9% and 14% inhibition of the activity at 1 and 10 mM, respectively Anabaena sp.
Mn2+ 57.1% inhibition at 5 mM Anabaena sp.
additional information the enzyme is not sensitive to the prototypical catalase inhibitor 3-amino-1, 2, 4,-triazole in presence of ascorbic acid (1.0 mM) Anabaena sp.
NaN3 82% inhibition 10 mM Anabaena sp.
phenylhydrazine complete inhibition at 1 mM Anabaena sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0036
-
 Reactive Blue 5 at pH 4.8 Anabaena sp.
0.0058
-
 H2O2 at pH 4.8 Anabaena sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53280
-
 4 * 53280, MALDI-TOF mass spectrometry Anabaena sp.
53368
-
 4 * 53368, calculated from amino acid sequence Anabaena sp.
54000
-
 4 * 54000, SDS-PAGE Anabaena sp.

Organism

Organism UniProt Comment Textmining
Anabaena sp.
-
 strain PCC 7120
-

Oxidation Stability

Oxidation Stability Organism
retains more than 50% of its activity at 2.5 mM H2O2 Anabaena sp.

Purification (Commentary)

Purification (Comment) Organism
DEAE-Toyopearl column chromatography, ammonium sulfate precipitation, butyl-Toyopearl column chromatography, and hydroxylapatite column chromatography Anabaena sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.6
-
 recombinant enzyme from crude extract Anabaena sp.
283
-
 recombinant enzyme after 50.5fold purification Anabaena sp.

Storage Stability

Storage Stability Organism
4°C, in 50 mM potassium phosphate (pH 7.0), 40 days, less than 10% loss of activity Anabaena sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,6-dimethoxyphenol + H2O2
-
 Anabaena sp. ?
-
 ?
4-aminoantipyrine + H2O2 643% activity compared to guaiacol Anabaena sp. ?
-
 ?
ABTS + H2O2 89% activity compared to guaiacol Anabaena sp. ?
-
 ?
Acid Blue 45 + H2O2
-
 Anabaena sp. ?
-
 ?
D-iso-ascorbate + H2O2 134% activity compared to guaiacol Anabaena sp. ?
-
 ?
guaiacol + H2O2 100% activity Anabaena sp. ?
-
 ?
L-ascorbate + H2O2 126% activity compared to guaiacol Anabaena sp. ?
-
 ?
additional information no activity with Mn2+ and veratryl alcohol as electron donors Anabaena sp. ?
-
 ?
NADH + H2O2 163% activity compared to guaiacol Anabaena sp. ?
-
 ?
NADPH + H2O2 134% activity compared to guaiacol Anabaena sp. ?
-
 ?
pyrogallol + H2O2 93% activity compared to guaiacol Anabaena sp. purpurogallin + H2O
-
 ?
Reactive Blue 114 + H2O2
-
 Anabaena sp. ?
-
 ?
Reactive Blue 119 + H2O2
-
 Anabaena sp. ?
-
 ?
Reactive Blue 4 + H2O2
-
 Anabaena sp. ?
-
 ?
Reactive Blue 5 + H2O2 123% activity compared to guaiacol, the apparent optimum concentration of H2O2 required for the decolorization of Reactive Blue 5 by AnaPX is 0.4 mM Anabaena sp. ?
-
 ?
syringaldehyde + H2O2 23% activity compared to guaiacol Anabaena sp. ?
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 54000, SDS-PAGE Anabaena sp.
homotetramer 4 * 53280, MALDI-TOF mass spectrometry Anabaena sp.
homotetramer 4 * 53368, calculated from amino acid sequence Anabaena sp.

Synonyms

Synonyms Comment Organism
AnaPX
-
 Anabaena sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Anabaena sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 60 the enzyme is considerably more stable at 30°C and 40°C where it retains more than 90% of its activity, the enzyme loses more than 90% of its activity after incubation at 50°C and 60°C for 3 h Anabaena sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 5
-
 Anabaena sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 9.5 when maintained at 40°C for 20 min, the enzyme is stable at pH values between 3.5 and 9.5 Anabaena sp.

Cofactor

Cofactor Comment Organism Structure
heme iron protoporphyrin-containing heme peroxidase Anabaena sp.

pI Value

Organism Comment pI Value Maximum pI Value
Anabaena sp. isoelectric focusing
-
 3.7