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Literature summary for 1.11.1.7 extracted from

  • Watanabe, L.; Nascimento, A.S.; Zamorano, L.S.; Shnyrov, V.L.; Polikarpov, I.
    Purification, crystallization and preliminary X-ray diffraction analysis of royal palm tree (Roystonea regia) peroxidase (2007), Acta Crystallogr. Sect. F, 63, 780-783.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.7

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method, with 2.6 M ammonium sulfate and 0.1 M MES, at pH 6.5 and 18°C Roystonea regia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 2 * 45000, gel filtration Roystonea regia
51000
-
 2 * 51000, SDS-PAGE Roystonea regia
90000
-
 gel filtration Roystonea regia
102000
-
 SDS-PAGE Roystonea regia

Organism

Organism UniProt Comment Textmining
Roystonea regia
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, phenyl-Sepharose column chromatography, DEAE-Toyopearl column chromatography, and Superdex 200 gel filtration Roystonea regia

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Roystonea regia
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 the unpurified homogenate has a specific activity of 18.84 units/mg, the 350fold purified enzyme has a specific activity of 6568.22 units/mg Roystonea regia

Subunits

Subunits Comment Organism
homodimer 2 * 51000, SDS-PAGE Roystonea regia
homodimer 2 * 45000, gel filtration Roystonea regia

Synonyms

Synonyms Comment Organism
royal palm tree peroxidase member of the class III peroxidases Roystonea regia
RPTP
-
 Roystonea regia

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 11
-
 Roystonea regia