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Literature summary for 1.11.1.7 extracted from

  • Perez-Boada, M.; Ruiz-Duenas, F.J.; Pogni, R.; Basosi, R.; Choinowski, T.; Martinez, M.J.; Piontek, K.; Martinez, A.T.
    Versatile peroxidase oxidation of high redox potential aromatic compounds: site-directed mutagenesis, spectroscopic and crystallographic investigation of three long-range electron transfer pathways (2005), J. Mol. Biol., 354, 385-402.
    View publication on PubMed
Search for more literature for 1.11.1.7

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pleurotus eryngii

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method. Crystallographic analysis of peroxide-treated wild-type enzyme and W164S variant Pleurotus eryngii

Protein Variants

Protein Variants Comment Organism
H232F kcat/Km for Reactive Black 5 is decreased, kcat/KM for veratryl alcohol is increased slightly. kcat/KM for Mn2+ as substrate is 1.5fold lower than the wild-type value Pleurotus eryngii
H232F mutant enzyme is completely unable to oxidize both veratryl, alcohol and Reactive Black 5. kcat/KM for Mn2+ as substrate is 1.6fold higher than the wild-type value Pleurotus eryngii
P76F kcat/KM for veratryl alcohol is increased slightly. kcat/KM for Mn2+ as substrate is 2.3fold lower than the wild-type value Pleurotus eryngii
W164S mutant enzyme is completely unable to oxidize both veratryl, alcohol and Reactive Black 5. kcat/KM for Mn2+ as substrate is 1.2fold higher than the wild-type value Pleurotus eryngii
W164S/P76H mutant enzyme is completely unable to oxidize both veratryl, alcohol and Reactive Black 5. kcat/KM for Mn2+ as substrate is 1.1fold lower than the wild-type value Pleurotus eryngii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0028
-
 Reactive Black 5 24°C, pH 3.5, wild-type enzyme Pleurotus eryngii
0.0031
-
 Reactive Black 5 24°C, pH 3.5, mutant enzyme P76H Pleurotus eryngii
0.0036
-
 Reactive Black 5 24°C, pH 3.5, mutant enzyme H232F Pleurotus eryngii
0.11
-
 Mn2+ 24°C, pH 5, mutant enzyme W164S Pleurotus eryngii
0.126
-
 Mn2+ 24°C, pH 5, mutant enzyme W164H Pleurotus eryngii
0.189
-
 Mn2+ 24°C, pH 5, wild-type enzyme Pleurotus eryngii
0.218
-
 Mn2+ 24°C, pH 5, mutant enzyme H232F Pleurotus eryngii
0.262
-
 Mn2+ 24°C, pH 5, mutant enzyme P76H Pleurotus eryngii
0.351
-
 Mn2+ 24°C, pH 5, mutant enzyme W164S/P76H Pleurotus eryngii
2.41
-
 veratryl alcohol 24°C, pH 3, mutant enzyme P76H Pleurotus eryngii
2.75
-
 veratryl alcohol 24°C, pH 3, wild-type enzyme Pleurotus eryngii
3.58
-
 veratryl alcohol 24°C, pH 3, mutant enzyme H232F Pleurotus eryngii

Organism

Organism UniProt Comment Textmining
Pleurotus eryngii O94753
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pleurotus eryngii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Mn2+ + H2O2
-
 Pleurotus eryngii ?
-
 ?
Reactive Black 5 + H2O2
-
 Pleurotus eryngii ?
-
 ?
veratryl alcohol + H2O2
-
 Pleurotus eryngii ?
-
 ?

Synonyms

Synonyms Comment Organism
versatile peroxidase
-
 Pleurotus eryngii
versatile peroxidase VPL2
-
 Pleurotus eryngii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.7
-
 Reactive Black 5 24°C, pH 3.5, mutant enzyme H232F Pleurotus eryngii
5
-
 Reactive Black 5 24°C, pH 3.5, wild-type enzyme Pleurotus eryngii
6
-
 Reactive Black 5 24°C, pH 3.5, mutant enzyme P76H Pleurotus eryngii
8
-
 veratryl alcohol 24°C, pH 3, wild-type enzyme Pleurotus eryngii
11
-
 veratryl alcohol 24°C, pH 3, mutant enzyme P76H Pleurotus eryngii
14
-
 veratryl alcohol 24°C, pH 3, mutant enzyme H232F Pleurotus eryngii
207
-
 Mn2+ 24°C, pH 5, mutant enzyme W164S Pleurotus eryngii
247
-
 Mn2+ 24°C, pH 5, mutant enzyme W164S/P76H Pleurotus eryngii
291
-
 Mn2+ 24°C, pH 5, mutant enzyme P76H Pleurotus eryngii
298
-
 Mn2+ 24°C, pH 5, wild-type enzyme Pleurotus eryngii
308
-
 Mn2+ 24°C, pH 5, mutant enzyme H232F Pleurotus eryngii
320
-
 Mn2+ 24°C, pH 5, mutant enzyme W164H Pleurotus eryngii