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Literature summary for 1.11.1.7 extracted from

  • Senczuk, A.M.; Machwe, A.; Kapoor, M.
    High constitutive peroxidase activity and constitutive thermotolerance in Neurospora crassa (2003), Mycoscience, 44, 129-137.
No PubMed abstract available
Search for more literature for 1.11.1.7

Inhibitors

Inhibitors Comment Organism Structure
Sodium azide 0.187 mM, more than 75% inhibition, 0.5 mM, complete inhibition, heme-containing enzyme Neurospora crassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) 25°C, pH 3.5 Neurospora crassa
0.055
-
 H2O2 25°C, pH 3.5 Neurospora crassa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
133000
-
 gel filtration Neurospora crassa

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose, metal chelate, superose-12 Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
reduced 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + H2O2
-
 Neurospora crassa oxidized 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + H2O
-
 ?

Synonyms

Synonyms Comment Organism
constitutive peroxidase
-
 Neurospora crassa
CP
-
 Neurospora crassa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
48 58 2-2.5fold increase in activity compared to 20°C Neurospora crassa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
52
-
 no loss of activity after 20 min Neurospora crassa
58
-
 no loss of activity after 20 min Neurospora crassa
62
-
 no loss of activity after 20 min Neurospora crassa
68
-
 approx. 50% loss of activity after 20 min Neurospora crassa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
 H2O2 25°C, pH 3.5 Neurospora crassa
0.3
-
 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) 25°C, pH 3.5 Neurospora crassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
-
 Neurospora crassa

pH Range

pH Minimum pH Maximum Comment Organism
2 5.5 approx. 30% of maximal activity at pH 2.0 Neurospora crassa

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 4 little loss of activity Neurospora crassa
5.5
-
 complete loss of activity Neurospora crassa