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Literature summary for 1.11.1.7 extracted from
- Rubrerythrin from the hyperthermophilic archaeon Pyrococcus furiosus is a rubredoxin-dependent, iron-containing peroxidase (2004), J. Bacteriol., 186, 7888-7895.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pyrococcus furiosus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.035 | - |
H2O2 | 85°C, pH 7.5 | Pyrococcus furiosus |  |
| 0.07 | - |
rubredoxin | 85°C, pH 7.5 | Pyrococcus furiosus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | 3 iron atoms per subunit, native enzyme | Pyrococcus furiosus | |
| Zinc | 2.5 zinc atoms and 0.5 iron atoms per subunit, recombinant enzyme from cells grown on M-Zn medium | Pyrococcus furiosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 19000 | - |
2 * 19000, SDS-PAGE | Pyrococcus furiosus |
| 19377 | - |
2 * 19377, mass spectroscopy | Pyrococcus furiosus |
| 36000 | - |
gel filtration | Pyrococcus furiosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native and recombinant enzyme | Pyrococcus furiosus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 36 | - |
at 85°C and pH 7.5 | Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| rubredoxin + H2O2 | - |
Pyrococcus furiosus | ? + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 19000, SDS-PAGE | Pyrococcus furiosus |
| dimer | 2 * 19377, mass spectroscopy | Pyrococcus furiosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| rubrerythrin | - |
Pyrococcus furiosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Pyrococcus furiosus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10.5 | approx. 60% of maximal activity at pH 6.0 and pH 8.0, approx. 30% of maximal activity at pH 9.5, almost no activity at pH 10.5 | Pyrococcus furiosus |
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Release 2023.1 (January 2023)
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