Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-aminothiazole | 2 mM, 4fold, more than 2fold and 1.4fold activation of 3,3',5,5'-tetramethylbenzidine, o-phenylenediamine and 5-aminosalycylic acid oxidation, respectively | Armoracia rusticana | |
melamine | 1 mM, approx. 2fold increase in kcat for oxidation of 3,3',5,5'-tetramethylbenzidine | Armoracia rusticana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4-dinitroresorcinol | competitive inhibition of 3,3',5,5'-tetramethylbenzidine oxidation | Armoracia rusticana | |
2-amino-4-nitrophenol | competitive inhibition of 3,3',5,5'-tetramethylbenzidine oxidation | Armoracia rusticana | |
gallic acid | competetive inhibition of 3,3',5,5'-tetramethylbenzidine oxidation | Armoracia rusticana | |
resorcinol | competitive inhibition of 3,3',5,5'-tetramethylbenzidine oxidation | Armoracia rusticana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
3,3',5,5'-tetramethylbenzidine | 20°C, pH 7.4 | Armoracia rusticana | |
0.09 | - |
3,3',5,5'-tetramethylbenzidine | 20°C, pH 6.8 | Armoracia rusticana | |
0.15 | - |
3,3',5,5'-tetramethylbenzidine | 20°C, pH 6.4 | Armoracia rusticana | |
0.38 | - |
o-phenylenediamine | 20°, pH 6.2 | Armoracia rusticana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Armoracia rusticana | - |
horseradish | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,3',5,5'-tetramethylbenzidine + H2O2 | - |
Armoracia rusticana | ? + H2O | - |
? | |
5-aminosalicylic acid + H2O2 | - |
Armoracia rusticana | ? + H2O | - |
? | |
o-phenylenediamine + H2O2 | - |
Armoracia rusticana | ? + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HP | acidic isoform, type A | Armoracia rusticana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
172 | - |
3,3',5,5'-tetramethylbenzidine | 20°C, pH 7.4 | Armoracia rusticana | |
435 | - |
3,3',5,5'-tetramethylbenzidine | 20°C, pH 6.8 | Armoracia rusticana | |
528 | - |
o-phenylenediamine | 20°, pH 6.2 | Armoracia rusticana | |
790 | - |
3,3',5,5'-tetramethylbenzidine | 20°C, pH 6.4 | Armoracia rusticana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
oxidation of 5-aminosalycylic acid | Armoracia rusticana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 7.5 | oxidation of 5-aminosalicylic acid, approx. 30% of maximal activity at pH 4.5 and pH 7.0, respectively | Armoracia rusticana |