Literature summary for 1.11.1.6 extracted from

Pei, J.; Wang, H.; Wu, L.; Xia, S.; Xu, C.; Zheng, B.; Li, T.; Jiang, Y.
Biochemical characterization of a catalase from Vibrio vulnificus, a pathogen that causes gastroenteritis (2017), Acta Biochim. Pol., 64, 543-549 .

Search for more literature for 1.11.1.6

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Vibrio vulnificus

Protein Variants

Protein Variants	Comment	Organism
H72A	mutant in heme-binding residue, almost complete loss of activity	Vibrio vulnificus
V71A and F158A	channel point mutant, about 20% of wild-type activity	Vibrio vulnificus
Y353A	mutant in heme-binding residue, almost complete loss of activity	Vibrio vulnificus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
10.5	-	H2O2	wild-type, pH 7.5, 35°C	Vibrio vulnificus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
240000	-	gel filtration	Vibrio vulnificus

Organism

Organism	UniProt	Comment	Textmining
Vibrio vulnificus	KTL38510	-	-
Vibrio vulnificus ATCC 43382	KTL38510	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 H2O2	-	Vibrio vulnificus	O2 + 2 H2O	-	?
2 H2O2	-	Vibrio vulnificus ATCC 43382	O2 + 2 H2O	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 57210, calculated, 4 * 58000, SDS-PAGE	Vibrio vulnificus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
35	-	-	Vibrio vulnificus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
37	-	2h, 80% residual activity	Vibrio vulnificus
45	-	40 min, 40% residual activity	Vibrio vulnificus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Vibrio vulnificus

Cofactor

Cofactor	Comment	Organism	Structure
heme	UV/visible spectrum shows a Soret peak at 407 nm	Vibrio vulnificus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Vibrio vulnificus	calculated	-	8.7

Expression

Organism	Comment	Expression
Vibrio vulnificus	transcription is upregulated by low salinity, heat, and oxidative stresses	up

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Release 2023.1 (January 2023)