Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain BL21 Star (DE3) | Mycothermus thermophilus |
Crystallization (Comment) | Organism |
---|---|
native and recombinant wild-type enzyme and enzyme mutants H82N and V123F, sitting drop vapour diffusion method, mixing of 9.4 mg/ml protein with reservoir solution containing 18% w/v PEG 2000, 0.1 M barium chloride, 0.1 M bis-tris pH 6.8 for the wild-type enzyme, and 6-16% w/v PEG 400, 0.2 M potassium chloride, 0.01 M calcium chloride, 0.05 M sodium cacodylate, pH 5.0-5.6 for the mutant H82N or PEG 4000 instead of PEG 400 for the mutant V123F, X-ray diffraction structure determination and analysis at 2.7 A, 1.4 A, 1.5 A, and 1.9 A resolution, respectively | Mycothermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
H82N | site-directed mutagenesis, the mutation results in conversion of the native d-type heme to a b-type heme | Mycothermus thermophilus |
V123F | site-directed mutagenesis | Mycothermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
79000 | - |
4 * 79000, wild-type and mutant enzymes, SDS-PAGE | Mycothermus thermophilus |
320000 | - |
- |
Mycothermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 H2O2 | Mycothermus thermophilus | - |
O2 + 2 H2O | - |
? | |
2 H2O2 | Mycothermus thermophilus ATCC 16454 | - |
O2 + 2 H2O | - |
? | |
additional information | Mycothermus thermophilus | the bifunctional catalase with phenol oxidase activity catalyses the decomposition of hydrogen peroxide into oxygen and water and also oxidizes various phenolic compounds | ? | - |
? | |
additional information | Mycothermus thermophilus ATCC 16454 | the bifunctional catalase with phenol oxidase activity catalyses the decomposition of hydrogen peroxide into oxygen and water and also oxidizes various phenolic compounds | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycothermus thermophilus | - |
gene catpo | - |
Mycothermus thermophilus ATCC 16454 | - |
gene catpo | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21 Star (DE3) to homogeneity by anion exchange chromatography and gel filtration | Mycothermus thermophilus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 H2O2 = O2 + 2 H2O | reaction mechanism, overview | Mycothermus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4 | - |
mutant H82N, pH 7.0, 60°C | Mycothermus thermophilus |
65 | - |
mutant V123F, pH 7.0, 60°C | Mycothermus thermophilus |
8870 | - |
native wild-type enzyme, pH 7.0, 60°C | Mycothermus thermophilus |
18710 | - |
recombinant wild-type enzyme, pH 7.0, 60°C | Mycothermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 H2O2 | - |
Mycothermus thermophilus | O2 + 2 H2O | - |
? | |
2 H2O2 | via Compound I and Compound II intermediates | Mycothermus thermophilus | O2 + 2 H2O | - |
? | |
2 H2O2 | - |
Mycothermus thermophilus ATCC 16454 | O2 + 2 H2O | - |
? | |
2 H2O2 | via Compound I and Compound II intermediates | Mycothermus thermophilus ATCC 16454 | O2 + 2 H2O | - |
? | |
additional information | the bifunctional catalase with phenol oxidase activity catalyses the decomposition of hydrogen peroxide into oxygen and water and also oxidizes various phenolic compounds | Mycothermus thermophilus | ? | - |
? | |
additional information | the bifunctional catalase with phenol oxidase activity catalyses the decomposition of hydrogen peroxide into oxygen and water and also oxidizes various phenolic compounds | Mycothermus thermophilus ATCC 16454 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 79000, wild-type and mutant enzymes, SDS-PAGE | Mycothermus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
CATPO | - |
Mycothermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Mycothermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Mycothermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the heme component is cis-hydroxychlorin gamma-spirolactone | Mycothermus thermophilus |
General Information | Comment | Organism |
---|---|---|
additional information | the heme-binding pocket contains two highly conserved water molecules on the distal side | Mycothermus thermophilus |