Literature summary for 1.11.1.6 extracted from

Shima, S.; Netrusov, A.; Sordel, M.; Wicke, M.; Hartmann, G.C.; Thauer, R.K.
Purification, characterization, and primary structure of a monofunctional catalase from Methanosarcina barkeri (1999), Arch. Microbiol., 171, 317-323.

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Inhibitors

Inhibitors	Comment	Organism	Structure
3-amino-1H-1,2,4-triazole	IC50: 80 mM	Methanosarcina barkeri
KCN	IC50: 0.005 mM	Methanosarcina barkeri
NaN3	IC50: 0.001 mM	Methanosarcina barkeri

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
54000	-	4 * 54000, SDS-PAGE	Methanosarcina barkeri
190000	-	gel filtration	Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
H2O2	Methanosarcina barkeri	monofunctional catalase	O2 + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Methanosarcina barkeri	O93662	-	-

Purification (Commentary)

Purification (Comment)	Organism
near homogeneity, chromatography techniques	Methanosarcina barkeri

Source Tissue

Source Tissue	Comment	Organism	Textmining
cell culture	-	Methanosarcina barkeri	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
61500	-	-	Methanosarcina barkeri

Storage Stability

Storage Stability	Organism
4°C, stable for one month	Methanosarcina barkeri

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
H2O2	monofunctional catalase	Methanosarcina barkeri	O2 + H2O	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 54000, SDS-PAGE	Methanosarcina barkeri

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
55	-	stable up to	Methanosarcina barkeri

Cofactor

Cofactor	Comment	Organism	Structure
heme	ferro-hemoprotein	Methanosarcina barkeri

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.001	-	IC50: 0.001 mM	Methanosarcina barkeri	NaN3
0.005	-	IC50: 0.005 mM	Methanosarcina barkeri	KCN
80	-	IC50: 80 mM	Methanosarcina barkeri	3-amino-1H-1,2,4-triazole

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Release 2023.1 (January 2023)