Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Geobacter sulfurreducens |
Crystallization (Comment) | Organism |
---|---|
sitting-drop vapor diffusion, 16% PEG 10000, 0.1 HEPES/NaOH, pH 7.4, 293K, wild-type enzyme, space group P1, 2.00 A resolution, mutant enzyme G94K/K97Q/R100I, space group P4321, 3.21 A resolution, mutant enzyme S134P/V135K, space group P21, 2.40 A resolution, mutant enzame S134P, space group P21, 2.40 A resolution | Geobacter sulfurreducens |
Protein Variants | Comment | Organism |
---|---|---|
A124K/K128A | site-directed mutagenesis, no significant changes | Geobacter sulfurreducens |
G94K/K97Q/R100I | site-directed mutagenesis, triple point mutant is created to mimic the critical loop region of, but its crystal structure reveals that the inactive, bishistidinyl-coordinated form of the active-site heme group is retained | Geobacter sulfurreducens |
S134P | site-directed mutagenesis, distortion of the loop region, accompanied by an opening of the active-site loop, leaving the enzyme in a constitutively active state | Geobacter sulfurreducens |
S134P/V135K | site-directed mutagenesis, distortion of the loop region, accompanied by an opening of the active-site loop, leaving the enzyme in a constitutively active state | Geobacter sulfurreducens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0062 | - |
H2O2 | pH5.5 | Geobacter sulfurreducens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferrocytochrome c + H2O2 | Geobacter sulfurreducens | - |
2 ferricytochrome c + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacter sulfurreducens | Q749D0 | DSM 12127 | - |
Purification (Comment) | Organism |
---|---|
chromatography | Geobacter sulfurreducens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
26.4 | - |
2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) and H2O2 as electron donor and acceptor | Geobacter sulfurreducens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferrocytochrome c + H2O2 | - |
Geobacter sulfurreducens | 2 ferricytochrome c + H2O | - |
? | |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Geobacter sulfurreducens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Geobacter sulfurreducens |
Synonyms | Comment | Organism |
---|---|---|
CcpA | bacterial di-heme cytochrome c peroxidase | Geobacter sulfurreducens |
cytochrome c peroxidase | - |
Geobacter sulfurreducens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.5 | - |
H2O2 | pH 5.5 | Geobacter sulfurreducens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Geobacter sulfurreducens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | - |
Geobacter sulfurreducens |
General Information | Comment | Organism |
---|---|---|
physiological function | bacterial di-heme cytochrome c peroxidases (CcpAs) protect the cell from reactive oxygen species by reducing hydrogen peroxide to water | Geobacter sulfurreducens |