Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21(DE3) | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
D18K | positive-to-negative charge-reversal mutant | Saccharomyces cerevisiae |
D210K | positive-to-negative charge-reversal mutant | Saccharomyces cerevisiae |
D33K | positive-to-negative charge-reversal mutant | Saccharomyces cerevisiae |
D34K | the mutation causes large increases in the Michaelis constant indicating a reduced affinity for cytochrome c | Saccharomyces cerevisiae |
D37K | positive-to-negative charge-reversal mutant | Saccharomyces cerevisiae |
E118K | positive-to-negative charge-reversal mutant | Saccharomyces cerevisiae |
E17K | positive-to-negative charge-reversal mutant | Saccharomyces cerevisiae |
E201K | positive-to-negative charge-reversal mutant | Saccharomyces cerevisiae |
E209K | positive-to-negative charge-reversal mutant | Saccharomyces cerevisiae |
E290K | positive-to-negative charge-reversal mutant | Saccharomyces cerevisiae |
E291K | positive-to-negative charge-reversal mutant | Saccharomyces cerevisiae |
E32K | positive-to-negative charge-reversal mutant | Saccharomyces cerevisiae |
E35K | positive-to-negative charge-reversal mutant | Saccharomyces cerevisiae |
E98K | positive-to-negative charge-reversal mutant | Saccharomyces cerevisiae |
K149D | positive-to-negative charge-reversal mutant | Saccharomyces cerevisiae |
R31E | positive-to-negative charge-reversal mutant | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | - |
iso-1 ferrocytochrome c | mutant enzyme D210K, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C | Saccharomyces cerevisiae | |
0.002 | - |
iso-1 ferrocytochrome c | mutant enzyme D18K, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C | Saccharomyces cerevisiae | |
0.0021 | - |
iso-1 ferrocytochrome c | recombinant wild type enzyme, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C | Saccharomyces cerevisiae | |
0.0023 | - |
iso-1 ferrocytochrome c | mutant enzyme E17K, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C | Saccharomyces cerevisiae | |
0.0024 | - |
iso-1 ferrocytochrome c | mutant enzyme D33K, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C | Saccharomyces cerevisiae | |
0.0028 | - |
iso-1 ferrocytochrome c | mutant enzyme E209K, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C | Saccharomyces cerevisiae | |
0.0029 | - |
iso-1 ferrocytochrome c | mutant enzyme E201K, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C | Saccharomyces cerevisiae | |
0.0031 | - |
iso-1 ferrocytochrome c | mutant enzyme E98K, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C | Saccharomyces cerevisiae | |
0.0035 | - |
iso-1 ferrocytochrome c | mutant enzyme E35K, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C | Saccharomyces cerevisiae | |
0.0038 | - |
iso-1 ferrocytochrome c | mutant enzyme E291K, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C | Saccharomyces cerevisiae | |
0.0045 | - |
iso-1 ferrocytochrome c | mutant enzyme E32K, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C | Saccharomyces cerevisiae | |
0.051 | - |
iso-1 ferrocytochrome c | mutant enzyme E118K, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C | Saccharomyces cerevisiae | |
0.06 | - |
iso-1 ferrocytochrome c | mutant enzyme E290K, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C | Saccharomyces cerevisiae | |
0.082 | - |
iso-1 ferrocytochrome c | mutant enzyme D37K, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C | Saccharomyces cerevisiae | |
0.1 | - |
iso-1 ferrocytochrome c | Km above 0.1 mM, mutant enzyme D34K, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C | Saccharomyces cerevisiae | |
0.1 | - |
iso-1 ferrocytochrome c | Km above 0.1 mM, mutant enzyme R31E, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
iso-1 ferrocytochrome c + H2O2 | - |
Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CCP | - |
Saccharomyces cerevisiae |