Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM of the cation in the assay solution inhibits the oxidation of horse cytochrome c but not Pseudomonas stutzeri cytochrome c551 | Pseudomonas stutzeri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | pre-incubation with the cation has no effect on activity | Pseudomonas stutzeri | |
additional information | catalytic center activity increases with ionic strength in the case of cytochrome c551, with horse heart cytochrome c, the catalytic center activity decreases exponentially | Pseudomonas stutzeri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
sedimentation velocity experiments, in the presence of EGTA | Pseudomonas stutzeri |
67000 | - |
sedimentation velocity experiments, untreated enzyme | Pseudomonas stutzeri |
80000 | - |
sedimentation velocity experiments, in the presence of Ca2+ | Pseudomonas stutzeri |
87000 | - |
sedimentation velocity experiments, at higher protein concentrations | Pseudomonas stutzeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ferrocytochrome c + H2O2 | Pseudomonas stutzeri | - |
ferricytochrome c + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas stutzeri | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel filtration and ion exchange chromatography | Pseudomonas stutzeri |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 ferrocytochrome c + H2O2 + 2 H+ = 2 ferricytochrome c + 2 H2O | pseudo-first-order kinetics | Pseudomonas stutzeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ferrocytochrome c + H2O2 | - |
Pseudomonas stutzeri | ferricytochrome c + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | ultracentrifugation, untreated enzyme | Pseudomonas stutzeri |
More | in the presence of added Ca2+ or higher protein concentrations, the enzyme partially shifts to a higher state of aggregation, presumably tetramer | Pseudomonas stutzeri |