Protein Variants | Comment | Organism |
---|---|---|
W191F | less efficient at catalytic turnover than the wild-type enzyme | Escherichia coli |
W51F | exhibits extensive dimerization | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Subunits | Comment | Organism |
---|---|---|
dimer | SDS-PAGE, wild type and W191F mutant enzymes | Escherichia coli |
heterodimer | SDS-PAGE, H2O2 oxidation induces heterodimerization between cytochrome c and both wild-type and W191F enzymes, but not with W51F mutant | Escherichia coli |
More | dimerization of wild-type enzyme is observed at H2O2/enzyme ratios of 3 and 10. W191F mutant dimerizes irrespectively of the H2O2/enzyme ratio. W51F mutant exhibits extensive dimerization on H2O2 oxidation and formation of higher molecular weight polymeric species indicating nonspecific crosslinking | Escherichia coli |
polymer | SDS-PAGE, W51F mutant | Escherichia coli |
trimer | SDS-PAGE, W51F mutant | Escherichia coli |