Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Neisseria gonorrhoeae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant with deletion of the translation start codon and 800 bp of the enzyme gene. Almost as active as the wild-type enzyme | Neisseria gonorrhoeae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
nitrite | 2 mM, in the presence of 0.88 M of H2O2, inhibits 50% enzyme activity | Neisseria gonorrhoeae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | the enzyme is a lipoprotein sarkosyl-soluble | Neisseria gonorrhoeae | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ferrocytochrome c + H2O2 | Neisseria gonorrhoeae | - |
ferricytochrome c + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neisseria gonorrhoeae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
inner membrane proteins solubilized in sarkosyl solution and precipitated with ethanol are purified by affinity chromatography | Neisseria gonorrhoeae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ferrocytochrome c + H2O2 | - |
Neisseria gonorrhoeae | ferricytochrome c + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | expression of two protein after induction of the enzyme expression, 45000 Da and 47000 Da, SDS-PAGE. The 45000 Da protein is solubilized in 0.1% sodium deoxycholate, which indicates that the protein is only loosely associated with the membrane. The 47000 Da protein is probably initially synthesized with a signal peptide that is later cleaved | Neisseria gonorrhoeae |