Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Synechocystis sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | complete inhibition at 1 mM | Synechocystis sp. | |
Cu2+ | complete inhibition at 1 mM | Synechocystis sp. | |
Mercaptosuccinate | - |
Synechocystis sp. | |
additional information | not inhibited by 1 mM cyanide and 1 mM azide | Synechocystis sp. | |
N-ethylmaleimide | 60% inhibition of isoform Gpx-1 at 1 mM | Synechocystis sp. | |
p-chloromercuribenzoate | 30% inhibition of isoform Gpx-1 at 0.3 mM | Synechocystis sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0573 | - |
NADPH | isoform Gpx-2, at pH 8.2 and 37°C | Synechocystis sp. | |
0.0821 | - |
alpha-linolenic acid hydroperoxide | isoform Gpx-2, at pH 8.2 and 37°C | Synechocystis sp. | |
0.083 | - |
NADPH | isoform Gpx-1, at pH 8.2 and 37°C | Synechocystis sp. | |
0.215 | - |
alpha-linolenic acid hydroperoxide | isoform Gpx-1, at pH 8.2 and 37°C | Synechocystis sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16600 | - |
isoform Gpx-2, gel filtration | Synechocystis sp. |
16645 | - |
1 * 16645, isoform Gpx-2, calculated from deduced amino acid sequence | Synechocystis sp. |
18400 | - |
isoform Gpx-1, gel filtration | Synechocystis sp. |
18451 | - |
1 * 18451, isoform Gpx-1, calculated from deduced amino acid sequence | Synechocystis sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ADP-Sepharose affinity column chromatography, gel filtration | Synechocystis sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.044 | - |
isoform Gpx-1, using oleic acid hydroperoxide as substrate, at pH 8.2 and 37°C | Synechocystis sp. |
0.053 | - |
isoform Gpx-2, using oleic acid hydroperoxide as substrate, at pH 8.2 and 37°C | Synechocystis sp. |
0.071 | - |
isoform Gpx-1, using cumene hydroperoxide as substrate, at pH 8.2 and 37°C | Synechocystis sp. |
0.08 | - |
isoform Gpx-2, using cumene hydroperoxide as substrate, at pH 8.2 and 37°C | Synechocystis sp. |
0.083 | - |
isoform Gpx-1, using tert-butyl hydroperoxide as substrate, at pH 8.2 and 37°C | Synechocystis sp. |
0.085 | - |
isoform Gpx-2, using tert-butyl hydroperoxide as substrate, at pH 8.2 and 37°C | Synechocystis sp. |
0.087 | - |
isoform Gpx-1, using alpha-linoleic acid hydroperoxide as substrate, at pH 8.2 and 37°C | Synechocystis sp. |
0.106 | - |
isoform Gpx-2, using alpha-linoleic acid hydroperoxide as substrate, at pH 8.2 and 37°C | Synechocystis sp. |
0.133 | - |
isoform Gpx-1, using alpha-linolenic acid hydroperoxide as substrate, at pH 8.2 and 37°C | Synechocystis sp. |
0.15 | - |
isoform Gpx-2, using alpha-linolenic acid hydroperoxide as substrate, at pH 8.2 and 37°C | Synechocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-linolenic acid hydroperoxide + NADPH + H+ | - |
Synechocystis sp. | ? + NADP+ + H2O | - |
? | |
cumene hydroperoxide + NADPH + H+ | - |
Synechocystis sp. | ? + NADP+ + H2O | - |
? | |
linoleic acid hydroperoxide + NADPH + H+ | - |
Synechocystis sp. | ? + NADP+ + H2O | - |
? | |
additional information | the enzyme does not use reduced glutathione as an electron donor. No activity with H2O2, phosphatidylcholine hydroperoxide and digalactosyl diacylglycerol hydroperoxide. Neither cytochrome c nor ascorbate substitute for NADPH as an electron donor | Synechocystis sp. | ? | - |
? | |
oleic acid hydroperoxide + NADPH + H+ | - |
Synechocystis sp. | oleic acid + NADP+ + H2O | - |
? | |
tert-butyl hydroperoxide + NADPH + H+ | - |
Synechocystis sp. | ? + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 16600, isoform Gpx-1, SDS-PAGE | Synechocystis sp. |
monomer | 1 * 16645, isoform Gpx-2, calculated from deduced amino acid sequence | Synechocystis sp. |
monomer | 1 * 18400, isoform Gpx-1, SDS-PAGE | Synechocystis sp. |
monomer | 1 * 18451, isoform Gpx-1, calculated from deduced amino acid sequence | Synechocystis sp. |
Synonyms | Comment | Organism |
---|---|---|
glutathione peroxidase-like protein-1 | - |
Synechocystis sp. |
glutathione peroxidase-like protein-2 | - |
Synechocystis sp. |
Gpx-1 | - |
Synechocystis sp. |
GPx-2 | - |
Synechocystis sp. |
slr1171 | - |
Synechocystis sp. |
slr1992 | - |
Synechocystis sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Synechocystis sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Synechocystis sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | dependent on | Synechocystis sp. |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.8 | - |
isoform Gpx-1, at pH 8.2 and 37°C | Synechocystis sp. | Mercaptosuccinate |