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Literature summary for 1.11.1.19 extracted from

  • Krahe, N.K.; Berger, R.G.; Ersoy, F.
    A DyP-Type peroxidase of Pleurotus sapidus with alkene cleaving activity (2020), Molecules, 25, 1536 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.19

Application

Application Comment Organism
additional information the enzyme can be used as biocatalyst for flavor production Pleurotus sapidus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Komagataella pfaffii GS115 cells Pleurotus sapidus

Inhibitors

Inhibitors Comment Organism Structure
H2O2 an increase of the hydrogen peroxide concentration of more than 0.1 mM leads to a continuous activity decrease Pleurotus sapidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.037
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at pH 4.0 and 30°C Pleurotus sapidus
1.025
-
 Mn2+ at pH 3.5 and 30°C Pleurotus sapidus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ highest activity in the presence of 25 mM Mn2+. The enzyme activity rises with increasing Mn2+ concentration, but is not completely dependent on the addition of Mn2+ Pleurotus sapidus

Organism

Organism UniProt Comment Textmining
Pleurotus sapidus A0A6H0MBW1
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap SP Sepharose column chromatography Pleurotus sapidus

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Pleurotus sapidus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-methyl isoeugenol + H2O2 + H+
-
 Pleurotus sapidus oxidized (E)-methyl isoeugenol + H2O
-
 ?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+
-
 Pleurotus sapidus oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O
-
 ?
alpha-methylstyrene + H2O2 + H+
-
 Pleurotus sapidus oxidized alpha-methylstyrene + H2O
-
 ?
annatto + H2O2 + H+
-
 Pleurotus sapidus oxidized annatto + H2O
-
 ?
beta-carotene + H2O2 + H+
-
 Pleurotus sapidus oxidized beta-carotene + H2O
-
 ?
Mn2+ + H2O2 + H+
-
 Pleurotus sapidus Mn3+ + 2 H2O
-
 ?
additional information the enzyme lacks the typical activity towards anthraquinone dyes like Reactive Blue 19 and Reactive Black 5 Pleurotus sapidus ?
-
-
trans-anethole + H2O2 + H+
-
 Pleurotus sapidus 4-anisaldehyde + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 61000, recombinant enzyme, SDS-PAGE Pleurotus sapidus
? x * 45000, native enzyme, SDS-PAGE Pleurotus sapidus
? x * 54900, SDS-PAGE, recombinant enzyme, calculated from amino acid sequence Pleurotus sapidus

Synonyms

Synonyms Comment Organism
dye-decolorizing peroxidase
-
 Pleurotus sapidus
DyP
-
 Pleurotus sapidus
DyP-type peroxidase
-
 Pleurotus sapidus
POX
-
 Pleurotus sapidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Pleurotus sapidus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 60 the enzyme is relatively stable at temperatures from 20 to 60°C with a residual activity of more than 80%. At higher temperatures, a high loss of activity is observed due to protein denaturation, resulting in residual activities of less than 5% Pleurotus sapidus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.8
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at pH 4.0 and 30°C Pleurotus sapidus
7.2
-
 Mn2+ at pH 3.5 and 30°C Pleurotus sapidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
-
 Pleurotus sapidus

pH Range

pH Minimum pH Maximum Comment Organism
3 5 more than 50% of activity is conserved between pH 3.0 and 5.0. At lower or higher pH values, less than 25% of activity remains Pleurotus sapidus

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 5.5 the enzyme activity is more than 90% between pH 2.0 and 5.5 after 1 h of incubation Pleurotus sapidus

Cofactor

Cofactor Comment Organism Structure
heme
-
 Pleurotus sapidus

pI Value

Organism Comment pI Value Maximum pI Value
Pleurotus sapidus isoelectric focusing
-
 6.7

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
7
-
 Mn2+ at pH 3.5 and 30°C Pleurotus sapidus
184
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at pH 4.0 and 30°C Pleurotus sapidus