Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLyS cells | Auricularia auricula-judae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | - |
Auricularia auricula-judae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
Reactive Black 5 | wild type enzyme, at pH 3.0 and 30°C | Auricularia auricula-judae | |
0.12 | - |
Reactive Blue 19 | wild type enzyme, at pH 3.5 and 30°C | Auricularia auricula-judae | |
0.283 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | wild type enzyme, at pH 3.0 and 30°C | Auricularia auricula-judae | |
1.32 | - |
2,6-dimethoxyphenol | wild type enzyme, at pH 3.0 and 30°C | Auricularia auricula-judae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Auricularia auricula-judae | I2DBY1 | - |
- |
Purification (Comment) | Organism |
---|---|
Q Sepharose column chromatography, Mono Q column chromatography, and Mono P column chromatography | Auricularia auricula-judae |
Renatured (Comment) | Organism |
---|---|
with oxidized glutathione (1.5 mM), hemin (0.03 mM) and urea (0.8 M) | Auricularia auricula-judae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | - |
Auricularia auricula-judae | ? | - |
? | |
2,6-dimethoxyphenol + H2O2 + H+ | - |
Auricularia auricula-judae | ? | - |
? | |
Reactive Black 5 + H2O2 + H+ | - |
Auricularia auricula-judae | ? | - |
? | |
Reactive Blue 19 + H2O2 + H+ | - |
Auricularia auricula-judae | ? | - |
? | |
veratryl alcohol + H2O2 + H+ | very weak substrate | Auricularia auricula-judae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47000, calculated from amino acid sequence | Auricularia auricula-judae |
Synonyms | Comment | Organism |
---|---|---|
dye-decolorizing peroxidase | - |
Auricularia auricula-judae |
DyP | - |
Auricularia auricula-judae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 65 | the wild type enzyme remains stable after 24 h at 30-55°C and shows 50% activity after 24 h at 65.5°C | Auricularia auricula-judae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.6 | - |
Reactive Black 5 | wild type enzyme, at pH 3.0 and 30°C | Auricularia auricula-judae | |
420 | - |
2,6-dimethoxyphenol | wild type enzyme, at pH 3.0 and 30°C | Auricularia auricula-judae | |
422 | - |
Reactive Blue 19 | wild type enzyme, at pH 3.5 and 30°C | Auricularia auricula-judae | |
654 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | wild type enzyme, at pH 3.0 and 30°C | Auricularia auricula-judae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | the enzyme shows more than 80% activity after 24 h at pH 4.0-9.0 | Auricularia auricula-judae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Auricularia auricula-judae | isoelectric focusing | - |
4.4 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
veratryl alcohol | wild type enzyme, at pH 2.5 and 30°C | Auricularia auricula-judae | |
320 | - |
2,6-dimethoxyphenol | wild type enzyme, at pH 3.0 and 30°C | Auricularia auricula-judae | |
400 | - |
Reactive Black 5 | wild type enzyme, at pH 3.0 and 30°C | Auricularia auricula-judae | |
2300 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | wild type enzyme, at pH 3.0 and 30°C | Auricularia auricula-judae | |
3600 | - |
Reactive Blue 19 | wild type enzyme, at pH 3.5 and 30°C | Auricularia auricula-judae |