KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.061 | - |
Acid Blue 324 | at pH 4.5 and 30°C | Streptomyces avermitilis | |
0.79 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at pH 4.5 and 30°C | Streptomyces avermitilis | |
1.46 | - |
2,6-dimethoxyphenol | at pH 4.0 and 30°C | Streptomyces avermitilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
gel filtration | Streptomyces avermitilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces avermitilis | - |
- |
- |
Streptomyces avermitilis ATCC31267 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 75 gel filtration | Streptomyces avermitilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.022 | - |
enzyme from supernatant, at pH 4.0 and 30°C | Streptomyces avermitilis |
3.08 | - |
after 140fold purification, at pH 4.0 and 30°C | Streptomyces avermitilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | - |
Streptomyces avermitilis | ? | - |
? | |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | - |
Streptomyces avermitilis ATCC31267 | ? | - |
? | |
2,6-dimethoxyphenol + H2O2 + H+ | - |
Streptomyces avermitilis | ? | - |
? | |
2,6-dimethoxyphenol + H2O2 + H+ | - |
Streptomyces avermitilis ATCC31267 | ? | - |
? | |
Acid Blue 324 + H2O2 + H+ | efficient substrate | Streptomyces avermitilis | ? | - |
? | |
Acid Blue 324 + H2O2 + H+ | efficient substrate | Streptomyces avermitilis ATCC31267 | ? | - |
? | |
Reactive Blue 19 + H2O2 + H+ | low activity | Streptomyces avermitilis | ? | - |
? | |
Reactive Blue 19 + H2O2 + H+ | low activity | Streptomyces avermitilis ATCC31267 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, SDS-PAGE | Streptomyces avermitilis |
Synonyms | Comment | Organism |
---|---|---|
DyP-type peroxidase | - |
Streptomyces avermitilis |
DyP2 | - |
Streptomyces avermitilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | the enzyme retains almost of its initial activity against ABTS and DMP after incubation at 30°C and 40°C for 120 min. The peroxidase activity significantly decreases after incubating at 50°C for 60 min, and is almost completely lost following incubation at 60°C for 30 min | Streptomyces avermitilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.097 | - |
2,6-dimethoxyphenol | at pH 4.0 and 30°C | Streptomyces avermitilis | |
0.78 | - |
Acid Blue 324 | at pH 4.5 and 30°C | Streptomyces avermitilis | |
1.21 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at pH 4.5 and 30°C | Streptomyces avermitilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 4.5 | - |
Streptomyces avermitilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Streptomyces avermitilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0668 | - |
2,6-dimethoxyphenol | at pH 4.0 and 30°C | Streptomyces avermitilis | |
1.71 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at pH 4.5 and 30°C | Streptomyces avermitilis | |
12.4 | - |
Acid Blue 324 | at pH 4.5 and 30°C | Streptomyces avermitilis |