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Literature summary for 1.11.1.19 extracted from

  • Sugawara, K.; Nishihashi, Y.; Narioka, T.; Yoshida, T.; Morita, M.; Sugano, Y.
    Characterization of a novel DyP-type peroxidase from Streptomyces avermitilis (2017), J. Biosci. Bioeng., 123, 425-430 .
    View publication on PubMed
Search for more literature for 1.11.1.19

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.061
-
 Acid Blue 324 at pH 4.5 and 30°C Streptomyces avermitilis
0.79
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at pH 4.5 and 30°C Streptomyces avermitilis
1.46
-
 2,6-dimethoxyphenol at pH 4.0 and 30°C Streptomyces avermitilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 gel filtration Streptomyces avermitilis

Organism

Organism UniProt Comment Textmining
Streptomyces avermitilis
-
-
-
Streptomyces avermitilis ATCC31267
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 75 gel filtration Streptomyces avermitilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.022
-
 enzyme from supernatant, at pH 4.0 and 30°C Streptomyces avermitilis
3.08
-
 after 140fold purification, at pH 4.0 and 30°C Streptomyces avermitilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Streptomyces avermitilis ?
-
 ?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Streptomyces avermitilis ATCC31267 ?
-
 ?
2,6-dimethoxyphenol + H2O2 + H+
-
 Streptomyces avermitilis ?
-
 ?
2,6-dimethoxyphenol + H2O2 + H+
-
 Streptomyces avermitilis ATCC31267 ?
-
 ?
Acid Blue 324 + H2O2 + H+ efficient substrate Streptomyces avermitilis ?
-
 ?
Acid Blue 324 + H2O2 + H+ efficient substrate Streptomyces avermitilis ATCC31267 ?
-
 ?
Reactive Blue 19 + H2O2 + H+ low activity Streptomyces avermitilis ?
-
 ?
Reactive Blue 19 + H2O2 + H+ low activity Streptomyces avermitilis ATCC31267 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 50000, SDS-PAGE Streptomyces avermitilis

Synonyms

Synonyms Comment Organism
DyP-type peroxidase
-
 Streptomyces avermitilis
DyP2
-
 Streptomyces avermitilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 60 the enzyme retains almost of its initial activity against ABTS and DMP after incubation at 30°C and 40°C for 120 min. The peroxidase activity significantly decreases after incubating at 50°C for 60 min, and is almost completely lost following incubation at 60°C for 30 min Streptomyces avermitilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.097
-
 2,6-dimethoxyphenol at pH 4.0 and 30°C Streptomyces avermitilis
0.78
-
 Acid Blue 324 at pH 4.5 and 30°C Streptomyces avermitilis
1.21
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at pH 4.5 and 30°C Streptomyces avermitilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 4.5
-
 Streptomyces avermitilis

Cofactor

Cofactor Comment Organism Structure
heme
-
 Streptomyces avermitilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0668
-
 2,6-dimethoxyphenol at pH 4.0 and 30°C Streptomyces avermitilis
1.71
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at pH 4.5 and 30°C Streptomyces avermitilis
12.4
-
 Acid Blue 324 at pH 4.5 and 30°C Streptomyces avermitilis