Literature summary for 1.11.1.19 extracted from

Rekik, H.; Nadia, Z.J.; Bejar, W.; Kourdali, S.; Belhoul, M.; Hmidi, M.; Benkiar, A.; Badis, A.; Sallem, N.; Bejar, S.; Jaouadi, B.
Characterization of a purified decolorizing detergent-stable peroxidase from Streptomyces griseosporeus SN9 (2015), Int. J. Biol. Macromol., 73, 253-263 .

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General Stability

General Stability	Organism
the enzyme shows high compatibility with EcoVax and Dipex as laundry detergents for 48 h at 40°C. The enzyme is highly stable against perfume and anti-redeposition agents, particularly 50 mM Na2CO3, and of cationic and other detergent compound, retaining 95, 104, 107, and 125% of its initial activity after treatment with 1% perfume, 50 mM hexadecyltrimethylammonium bromide, 1% (w/v) sodium tripolyphosphate, and 50 mM Na2CO3, respectively.	Streptomyces griseosporeus

Organism

the enzyme shows high compatibility with EcoVax and Dipex as laundry detergents for 48 h at 40°C. The enzyme is highly stable against perfume and anti-redeposition agents, particularly 50 mM Na2CO3, and of cationic and other detergent compound, retaining 95, 104, 107, and 125% of its initial activity after treatment with 1% perfume, 50 mM hexadecyltrimethylammonium bromide, 1% (w/v) sodium tripolyphosphate, and 50 mM Na2CO3, respectively.

Streptomyces griseosporeus

Inhibitors

Inhibitors	Comment	Organism
Ba2+	about 85% inhibition at 1 mM	Streptomyces griseosporeus
Cd2+	complete inhibition at 1 mM	Streptomyces griseosporeus
Co2+	about 56% inhibition at 1 mM	Streptomyces griseosporeus
Hg2+	complete inhibition at 1 mM	Streptomyces griseosporeus
potassium cyanide	13% inhibition at 1 mM	Streptomyces griseosporeus
Sodium azide	9% inhibition at 1 mM	Streptomyces griseosporeus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.112	-	2,4-Dichlorophenol	at pH 8.5 and 25°C	Streptomyces griseosporeus
0.458	-	o-Dianisidine	at pH 8.5 and 25°C	Streptomyces griseosporeus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Streptomyces griseosporeus	-	-

Metals/Ions

Metals/Ions	Comment	Organism
Ca2+	about 116% activity at 1 mM	Streptomyces griseosporeus
Mn2+	about 139% activity at 1 mM. The addition of 1 mM of Mn2+ also enhances the thermostability of the enzyme	Streptomyces griseosporeus
additional information	Cu2+, Mg2+, Zn2+, and Fe2+ do not exert significant effects on enzyme activity	Streptomyces griseosporeus

Organism

Organism	UniProt	Comment	Textmining
Streptomyces griseosporeus	-	-	-
Streptomyces griseosporeus SN9	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
OH-40 column chromatography and UNO Q-6 column chromatography	Streptomyces griseosporeus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2	about 92% activity compared to 2,4-dichlorophenol	Streptomyces griseosporeus	?	-	?
2,4,6-trichlorophenol + H2O2 + H+	about 63% activity compared to 2,4-dichlorophenol	Streptomyces griseosporeus	?	-	?
2,4-dichlorophenol + H2O2 + H+	100% activity	Streptomyces griseosporeus	?	-	?
2,6-dimethoxyphenol + H2O2 + H+	-	Streptomyces griseosporeus	?	-	?
4-chlorophenol + H2O2 + H+	about 45% activity compared to 2,4-dichlorophenol	Streptomyces griseosporeus	?	-	?
Acid Blue 158 + H2O2 + H+	-	Streptomyces griseosporeus	?	-	?
Cibacet Brilliant Blue BG + H2O2 + H+	-	Streptomyces griseosporeus	?	-	?
guaiacol + H2O2 + H+	about 27% activity compared to 2,4-dichlorophenol	Streptomyces griseosporeus	?	-	?
additional information	no activity with commercial humic acid	Streptomyces griseosporeus	?	-	?
o-dianisidine + H2O2 + H+	about 76% activity compared to 2,4-dichlorophenol	Streptomyces griseosporeus	?	-	?
pyrogallol + H2O2 + H+	about 75% activity compared to 2,4-dichlorophenol	Streptomyces griseosporeus	?	-	?
Remazol Brilliant Blue Reactif + H2O2 + H+	-	Streptomyces griseosporeus	?	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 43000, SDS-PAGE	Streptomyces griseosporeus

Synonyms

Synonyms	Comment	Organism
LiP-SN	-	Streptomyces griseosporeus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
65	-	-	Streptomyces griseosporeus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	the half-life at 60°C is 120 min	Streptomyces griseosporeus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0635	-	2,4-Dichlorophenol	at pH 8.5 and 25°C	Streptomyces griseosporeus
1.17	-	o-Dianisidine	at pH 8.5 and 25°C	Streptomyces griseosporeus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	-	Streptomyces griseosporeus

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
7	10	the enzyme maintains 100% of its original activity at pH values between 7 and 10 after incubation for 48 h at 40°C	Streptomyces griseosporeus

Cofactor

Cofactor	Comment	Organism	Structure
heme	-	Streptomyces griseosporeus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.567	-	2,4-Dichlorophenol	at pH 8.5 and 25°C	Streptomyces griseosporeus
2.54	-	o-Dianisidine	at pH 8.5 and 25°C	Streptomyces griseosporeus