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Literature summary for 1.11.1.19 extracted from
- A dye-decolorizing peroxidase from Vibrio cholerae (2015), Biochemistry, 54, 6610-6621 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Vibrio cholerae |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| sitting drop vapor diffusion method, using 0.2 M potassium thiocyanate and 20% (w/v) PEG 3000 | Vibrio cholerae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
guaiacol | at pH 8.0 and 25°C | Vibrio cholerae |  |
| 0.05 | - |
Reactive Blue 19 | at pH 8.0 and 25°C | Vibrio cholerae | |
| 0.18 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at pH 8.0 and 25°C | Vibrio cholerae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
gel filtration | Vibrio cholerae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio cholerae | Q9KQ59 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HisTrap column chromatography and Superdex 200 gel filtration | Vibrio cholerae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | - |
Vibrio cholerae | ? | - |
? | |
| guaiacol + H2O2 + H+ | - |
Vibrio cholerae | ? | - |
? | |
| Reactive Blue 19 + H2O2 + H+ | - |
Vibrio cholerae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 35000, SDS-PAGE | Vibrio cholerae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dye-decolorizing peroxidase | - |
Vibrio cholerae |
| DyP | - |
Vibrio cholerae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.027 | - |
guaiacol | at pH 8.0 and 25°C | Vibrio cholerae | |
| 1.3 | - |
Reactive Blue 19 | at pH 8.0 and 25°C | Vibrio cholerae | |
| 500 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | at pH 8.0 and 25°C | Vibrio cholerae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Vibrio cholerae |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 26 | - |
Reactive Blue 19 | at pH 8.0 and 25°C | Vibrio cholerae | |
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Release 2023.1 (January 2023)
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