Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Corallina officinalis |
Protein Variants | Comment | Organism |
---|---|---|
H480A | results in the loss of the ability to efficiently oxidize bromide, but retains the ability to oxidize iodide | Corallina officinalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
Br- | pH 6.5, recombinant enzyme | Corallina officinalis | |
0.0013 | - |
Br- | pH 6.5, native enzyme | Corallina officinalis | |
0.017 | - |
H2O2 | pH 6.5, recombinant enzyme | Corallina officinalis | |
0.027 | - |
H2O2 | pH 6.5, native enzyme | Corallina officinalis | |
0.2 | - |
H2O2 | pH 6.5, mutant enzyme H480A | Corallina officinalis | |
1.8 | - |
I- | recombinant enzyme | Corallina officinalis | |
33 | - |
I- | mutant enzyme H480A | Corallina officinalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corallina officinalis | Q8LLW7 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Corallina officinalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione | i.e. monochlorodimedone | Corallina officinalis | ? | - |
? | |
I- + H2O2 | - |
Corallina officinalis | triiodide + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CVBPO | - |
Corallina officinalis |
vanadium bromoperoxidase | - |
Corallina officinalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | maximum bromoperoxidase activity of mutant H480A under conditions of high substrate concentrations (5 mM H2O2 and 100 mM Br-), however with reduced specific activity as compared to recombinant wild-type enzyme | Corallina officinalis |
6 | - |
optimal bromoperoxidase activity of recombinant wild-type enzyme using low substrate concentrations (0.5 mM H2O2, 5 mM Br-) and high substrate concentrations (5 mM H2O2, 100 mM Br-) | Corallina officinalis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | pH 5.0: about 35% of maximal activity, pH 7.0: about 25% of maximal activity | Corallina officinalis |