Crystallization (Comment) | Organism |
---|---|
structural changes in the mutants D153H, D153A, R244L, N246H, N246A, D153A/N246A are confined to the distal heme environment | Rhodococcus jostii |
Protein Variants | Comment | Organism |
---|---|---|
D153A | mutation minimally affects the second order rate constant for Compound I formation and the specificity constant for H2O2, but substitution dramatically reduces the stability of Compound I | Rhodococcus jostii |
D153A/N246A | mutation reduces the second order rate constant for Compound I formation and the specificity constant for H2O2 less than 30fold, substitution dramatically reduces the stability of Compound I | Rhodococcus jostii |
D153H | mutant is more than an order of magnitude less reactive with H2O2 than wild-type | Rhodococcus jostii |
N246A | mutation inimally affects the second order rate constant for Compound I formation and the specificity constant for H2O2, but substitution dramatically reduces the stability of Compound I | Rhodococcus jostii |
N246H | no detectable peroxidase activity | Rhodococcus jostii |
R244L | mutation abolishes the peroxidase activity, and heme iron of the mutant shows a pH-dependent transition from high spin pH 5 to low spin pH 8.5 | Rhodococcus jostii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
H2O2 | mutant N246A, pH 5.5, 25°C | Rhodococcus jostii | |
0.027 | - |
H2O2 | wild-type, pH 5.5, 25°C | Rhodococcus jostii | |
0.087 | - |
H2O2 | mutant D153H, pH 5.5, 25°C | Rhodococcus jostii | |
0.46 | - |
H2O2 | mutant D153A, pH 5.5, 25°C | Rhodococcus jostii | |
5 | - |
H2O2 | mutant D153A/N246A, pH 5.5, 25°C | Rhodococcus jostii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus jostii | Q0SE24 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+ | - |
Rhodococcus jostii | ? + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
B-type dye-decolorizing peroxidase | - |
Rhodococcus jostii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
H2O2 | mutant D153H, pH 5.5, 25°C | Rhodococcus jostii | |
3.2 | - |
H2O2 | wild-type, pH 5.5, 25°C | Rhodococcus jostii | |
5.4 | - |
H2O2 | mutant N246A, pH 5.5, 25°C | Rhodococcus jostii | |
19 | - |
H2O2 | mutant D153A/N246A, pH 5.5, 25°C | Rhodococcus jostii | |
51 | - |
H2O2 | mutant D153A, pH 5.5, 25°C | Rhodococcus jostii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Rhodococcus jostii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4 | - |
H2O2 | mutant D153A/N246A, pH 5.5, 25°C | Rhodococcus jostii | |
20 | - |
H2O2 | mutant D153H, pH 5.5, 25°C | Rhodococcus jostii | |
110 | - |
H2O2 | mutant D153A, pH 5.5, 25°C | Rhodococcus jostii | |
120 | - |
H2O2 | wild-type, pH 5.5, 25°C | Rhodococcus jostii | |
360 | - |
H2O2 | mutant N246A, pH 5.5, 25°C | Rhodococcus jostii |