Any feedback?
Literature summary for 1.11.1.16 extracted from
- Expression of soluble versatile peroxidase of Bjerkandera adusta in Escherichia coli (2009), Biores. Technol., 100, 851-858.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | production of the oxidase in strain BL21(DE3)pLysS, cultivated at 25°C in auto-induction medium and presence of heme | Bjerkandera adusta |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bjerkandera adusta |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| construction of enzyme model and identification of active sites for oxidation of Mn2+ and of aromatic substrates | Bjerkandera adusta |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
veratryl alcohol | pH 2.5, 30°C | Bjerkandera adusta |  |
| 70 | - |
1-naphthol | pH 2.5, 30°C | Bjerkandera adusta | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Bjerkandera adusta | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | - |
Bjerkandera adusta | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36400 | - |
x * 36400, calculated | Bjerkandera adusta |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bjerkandera adusta | A5JTV4 | fragment | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | sequence has one potential N-glycosylation site at Asn109 | Bjerkandera adusta |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Bjerkandera adusta |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-naphthol + H2O2 | - |
Bjerkandera adusta | ? + H2O | - |
? | |
| veratryl alcohol + H2O2 + H+ | - |
Bjerkandera adusta | veratraldehyde + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 36400, calculated | Bjerkandera adusta |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 79 | - |
melting temperature | Bjerkandera adusta |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bjerkandera adusta | calculated | - |
4.2 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
