Any feedback?
Literature summary for 1.11.1.14 extracted from
- Characterization of purified and xerogel immobilized novel lignin peroxidase produced from Trametes versicolor IBL-04 using solid state medium of corncobs (2012), BMC Biotechnol., 12, 46.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | immobilization of enzyme by entrapping in xerogel matrix of trimethoxysilane and proplytetramethoxysilane to maximum immobilization efficiency of 88.6%. The free and immobilized enzymes have optimum pH values of 6 and 5 while optimum temperatures are 60°C and 80°C, respectively. Immobilization enhances the activity and thermo-stability potential significantly and immobilized enzyme remains stable over broad pH and temperature range | Trametes versicolor |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 1 mM, about 80% inhibition of native enzyme, about 15% inhibition of enzyme immobilized in xerogel matrix of trimethoxysilane and proplytetramethoxysilane | Trametes versicolor |  |
| EDTA | 1 mM, about 75% inhibition of native enzyme, about 20% inhibition of enzyme immobilized in xerogel matrix of trimethoxysilane and proplytetramethoxysilane | Trametes versicolor | |
| L-cysteine | 1 mM, complete inhibition of native enzyme, about 30% inhibition of enzyme immobilized in xerogel matrix of trimethoxysilane and proplytetramethoxysilane | Trametes versicolor | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
veratryl alcohol | native enzyme, 25°C, pH 6.0 | Trametes versicolor | |
| 0.588 | - |
veratryl alcohol | enzyme immobilized in xerogel matrix of trimethoxysilane and proplytetramethoxysilane, 25°C, pH 5.0 | Trametes versicolor | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 1 mM, slight stimulation | Trametes versicolor | |
| Fe2+ | 1 mM, slight stimulation | Trametes versicolor | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
PAGE | Trametes versicolor |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trametes versicolor | - |
- |
- |
| Trametes versicolor IBL-04 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Trametes versicolor |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 553 | - |
pH 6.0, 25°C | Trametes versicolor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| veratryl alcohol + H2O2 | - |
Trametes versicolor | 3,4-dimethoxybenzaldehyde + H2O | - |
? | |
| veratryl alcohol + H2O2 | - |
Trametes versicolor IBL-04 | 3,4-dimethoxybenzaldehyde + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 30000, SDS-PAGE | Trametes versicolor |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LIP | - |
Trametes versicolor |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
native enzyme | Trametes versicolor |
| 80 | - |
enzyme immobilized in xerogel matrix of trimethoxysilane and proplytetramethoxysilane | Trametes versicolor |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
enzyme immobilized in xerogel matrix of trimethoxysilane and proplytetramethoxysilane | Trametes versicolor |
| 6 | - |
native enzyme | Trametes versicolor |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6 | native enzyme, stable for 1 h, enzyme immobilized in xerogel matrix of trimethoxysilane and proplytetramethoxysilane, stable for 24 h | Trametes versicolor |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
